کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8841058 | 1614705 | 2018 | 26 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Longitudinal Diffusion Tensor Imaging Revealed Nerve Fiber Alterations in Aspm Mutated Microcephaly Model Mice
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
In vivo MRICNTMBPASPMCorpus callosumλ1TBSSCingulumDTITFCEaxial diffusivity - diffusivity محوریtract-based spatial statistics - آمار مکانی مبتنی بر تراکمthreshold-free cluster enhancement - افزایش خوشه ای آستانه ایradial diffusivity - انتشار شعاعیlateral ventricle - بطن جانبیdiffusion tensor imaging - تصویربرداری تانسور انتشارNerve fiber - فیبر عصبیcerebral cortex - قشر مغزHIP - مفصل ران یا مفصل هیپmicrocephaly - میکروسفالیfractional anisotropy - ناپیوستگی کسریknockout - ناکاوتHistopathology - هیستوپاتولوژیHippocampus - هیپوکامپ Myelin basic protein - پروتئین پایه میلینanterior commissure - کمربند قدامیControl - کنترلexternal capsule - کپسول خارجی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Autosomal recessive primary microcephaly-5 (MCPH5) is characterized by congenital microcephaly and is caused by the mutation in the abnormal spindle-like, microcephaly-associated (ASPM) gene. This study aimed to demonstrate a correlation between radiological and pathological analyses in evaluating postnatal brain development using MCPH5-model mice, ASPM ortholog (Aspm) knockout (KO) mice. In vivo MRI was performed at two time points (postnatal 3â¯weeks; P3W and P10W) and complementary histopathological analyses of brains were done at P5W and P13W. In the MRI analysis, Aspm KO mice showed significantly decreased brain sizes (average 8.6% difference) with larger ventricles (average 136.4% difference) at both time points. Voxel-based statistics showed that the fractional anisotropy (FA) values were significantly lower in Aspm KO mice in both the cortex and white matter at both time points. Developmental changes in the FA values were less remarkable in the Aspm KO mice, compared with the controls. Histometric analyses revealed that the ratios of the horizontal to the vertical neurites were significantly higher in cortical layers IV, V and VI, with a remarkable increase according to maturation at P13W in the control mice (average 12.7% difference between control and KO), whereas the ratio in layer VI decreased at P13W in the KO mice. The myelin basic protein positive ratio in the white matter significantly decreased in Aspm KO mice at P5W. These results suggest that temporal FA changes are closely correlated with pathological findings such as abnormal neurite outgrowth and differentiation, which may be applicable for analyzing diseased human brain development.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 371, 10 February 2018, Pages 325-336
Journal: Neuroscience - Volume 371, 10 February 2018, Pages 325-336
نویسندگان
Hiroshi Ogi, Nobuhiro Nitta, So Tando, Akira Fujimori, Ichio Aoki, Shinji Fushiki, Kyoko Itoh,