کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8841291 | 1614711 | 2017 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Acylated and unacylated ghrelin confer neuroprotection to mesencephalic neurons
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
VGCCacylatedMDAgrowth hormone secretagogue receptor 1AGHSR1aUAGOCRCCCP3-NPNeuNMPP+ - MPP +Goat - بزParkinson disease - بیماری پارکینسونMitochondrial respiratory chain - زنجیره تنفسی mitochondrialBBB - سد خونی مغزیblood–brain-barrier - مانع خون مغزیOxygen consumption rates - میزان مصرف اکسیژنneuronal nuclei - هسته های نورونیvoltage-gated Ca2+ channels - کانال های Ca2 + دارای ولتاژcarbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone - کربونیل سیانید 4- (trifluoromethoxy) phenylhydrazoneGhrelin - گرلینGhrelin O-acyltransferase - گرلین O-acyltransferaseGhrelin receptor - گیرنده گرلین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The polypeptide ghrelin is an endogenous ligand at the growth hormone secretagogue receptor 1a. To ghrelin multiple functions have been ascribed including promotion of gastrointestinal motility. Postprandial ghrelin levels have been reported to be reduced in patients suffering from Parkinson disease (PD). Experimental studies revealed neuroprotective effects of ghrelin in different PD models. The purpose of the present study was (i) to further elucidate the mechanism underlying the neuroprotective action of ghrelin and (ii) to determine whether these effects occur with both the acylated and the unacylated form. The study was conducted in primary mesencephalic cultures treated with mitochondrial complex I and complex II inhibitors. We show that protective effects of ghrelin against complex I inhibition with MPP+ were independent of the acylation status of ghrelin, although acylated ghrelin appeared to be more potent. Protection by both forms was also observed when neurons were exposed to the complex II inhibitor 3-NP. Both forms led to higher oxygen consumption rates upon electron transport chain uncoupling, indicating that the two peptides may exert uncoupling effects themselves. We demonstrate that the rescue provided by ghrelin required calcium influx through L-type voltage-gated calcium channels. Whereas the protective effects of acylated ghrelin required receptor binding, effects of the unacylated form remained unaffected by treatment with a ghrelin receptor antagonist. Importantly, inhibition of ghrelin O-acyltransferase failed to reduce the activity of unacylated ghrelin. Overall, our data suggest that both acylated and unacylated ghrelin afford protection to dopamine neurons but through mechanisms that only partially overlap.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 365, 4 December 2017, Pages 137-145
Journal: Neuroscience - Volume 365, 4 December 2017, Pages 137-145
نویسندگان
Johanna Wagner, Franca VulinoviÄ, Anne Grünewald, Marcus M. Unger, Jens C. Möller, Christine Klein, Patrick P. Michel, Vincent Ries, Wolfgang H. Oertel, Daniel Alvarez-Fischer,