کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8843855 1616288 2018 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An improved test for the evaluation of hydrocarbon degradation capacities of diesel-contaminating microorganisms
ترجمه فارسی عنوان
یک آزمایش پیشرفته برای ارزیابی ظرفیت تخریب هیدروکربن میکروارگانیسم های آلوده کننده دیزلی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست علوم زیست محیطی (عمومی)
چکیده انگلیسی
The development of a test to evaluate the degradation of semi-volatile fuels as diesel by microorganisms is presented. This method is based on the principles described in the CEC-L-103 standard procedure that is exclusively meant for testing the biodegradability of non-volatile lubricants. Therefore, significant modifications involve aseptic conditions for testing specific microorganisms and conducting the test in closed vessels avoiding evaporation losses, while fuel quantification using gas chromatography-flame ionization detection (GC-FID) is retained. It is suggested that the modified procedure should enable routine application for semi-volatile hydrocarbon-based fuels. GC-FID provides additionally valuable information on the alteration of fuel component patterns during biodegradation. The procedure was successfully tested using two bacteria (Pseudomonas aeruginosa and Sphingomonas sp.) and two yeasts (Moesziomyces sp. and Candida sp.) isolated from real diesel contamination cases. All tested microorganisms caused a significant degradation of diesel fuel achieving hydrocarbon degradation percentages ranging from 23% to 35%. Specific aspects on the test modification and prospects for further modification regarding targeted investigations in the field of fuel contamination by microorganisms are briefly discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Biodeterioration & Biodegradation - Volume 129, April 2018, Pages 89-94
نویسندگان
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