کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8850079 | 1618663 | 2017 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Computational identification and characterization of miRNAs and their target genes from five cyprinidae fishes
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بوم شناسی
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چکیده انگلیسی
MicroRNAs (miRNAs) are a kind of small single-strand RNA molecules with lengths of 18-25Â nt, which do not encode any proteins. They play an essential role in gene expression regulation by binding to their target genes, leading to translational repression or transcript degradation. In this study, 23 miRNAs were predicted from five cyprinidae fishes by using a bioinformatics-based gene search based on blasting ESTs and GSS in NCBI, of which 21 miRNA genes have not been previously reported. To prove their validity, five of the computationally predicted miRNAs were verified by RTPCR, their transcripts were successfully detected, and, 46 potential target genes for these miRNAs were predicted, most target genes encode transcription factors, they are involved in signal transduction, metabolism and development processes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Saudi Journal of Biological Sciences - Volume 24, Issue 6, September 2017, Pages 1126-1135
Journal: Saudi Journal of Biological Sciences - Volume 24, Issue 6, September 2017, Pages 1126-1135
نویسندگان
Yong Huang, Hong-Tao Ren, Quan Zou, Yu-Qin Wang, Ji-Liang Zhang, Xue-Li Yu,