Spirulina platensis attenuates furan reprotoxicity by regulating oxidative stress, inflammation, and apoptosis in testis of rats

Furan is a common food contaminant and environmental pollutant. Spirulina platensis (SP) is a blue-green algae extensively used as therapeutic and health supplements. This study aimed to explore the probable beneficial role of SP against the influence of furan on reproductive system of male rats. Adult male rats were divided into control, vehicle control, SP (300 mg/kg bwt/ day, 7 days), furan (16 mg/kg bwt/ day,30 day), SP/furan, furan/SP and furan+SP groups. Hematology, sperm count, sperm morphology, serum testosterone (TES), luteinizing hormone (LH), follicle-stimulating hormone (FSH) and estradiol (E2) levels, reduced glutathione (GSH), malondialdehyde (MDA), testicular enzymes, and pro inflammatory cytokines were estimated. In addition, histopathology of testis and seminal vesicles and apoptosis were evaluated. Anaemia, leukocytosis, and reduced gonadosomatic index were observed in the furan treated group. TES, LH, FSH, E2, and GSH were significantly decreased following furan treatment. MDA, testicular enzymes, and pro inflammatory cytokines were significantly incremented in testis of furan treated rats. Furan induced apoptic changes in testis. SP significantly counteracted furan reprotoxic impacts, particularly at co-exposure. Conclusively, these findings verified that SP could be candidate therapy against furan reprotoxic impacts.