کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8854051 | 1618910 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Gamma irradiation during gametogenesis in young adult zebrafish causes persistent genotoxicity and adverse reproductive effects
ترجمه فارسی عنوان
اشعه گاما در طی گامتوژنز در سوپروز ماهیان جوان باعث سمیت ژنتیکی و اثرات جانبی ناشی از تولید مثل می شود
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
شیمی زیست محیطی
چکیده انگلیسی
The biological effects of gamma radiation may exert damage beyond that of the individual through its deleterious effects on reproductive function. Impaired reproductive performance can result in reduced population size over consecutive generations. In a continued effort to investigate reproductive and heritable effects of ionizing radiation, we recently demonstrated adverse effects and genomic instability in progeny of parents exposed to gamma radiation. In the present study, genotoxicity and effects on the reproduction following subchronic exposure during a gametogenesis cycle to 60Co gamma radiation (27 days, 8.7 and 53â¯mGy/h, total doses 5.2 and 31â¯Gy) were investigated in the adult wild-type zebrafish (Danio rerio). A significant reduction in embryo production was observed one month after exposure in the 53â¯mGy/h exposure group compared to control and 8.7â¯mGy/h. One year later, embryo production was significantly lower in the 53â¯mGy/h group compared only to control, with observed sterility, accompanied by a regression of reproductive organs in 100% of the fish 1.5 years after exposure. Histopathological examinations revealed no significant changes in the testis in the 8.7â¯mGy/h group, while in 62.5% of females exposed to this dose rate the oogenesis was found to be only at the early previtellogenic stage. The DNA damage determined in whole blood, 1.5 years after irradiation, using a high throughput Comet assay, was significantly higher in the exposed groups (1.2 and 3-fold increase in 8.7 and 53â¯mGy/h females respectively; 3-fold and 2-fold increase in 8.7 and 53â¯mGy/h males respectively) compared to controls. A significantly higher number of micronuclei (4-5%) was found in erythrocytes of both the 8.7 and 53â¯mGy/h fish compared to controls. This study shows that gamma radiation at a dose rate of â¥â¯8.7â¯mGy/h during gametogenesis causes adverse reproductive effects and persistent genotoxicity (DNA damage and increased micronuclei) in adult zebrafish.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ecotoxicology and Environmental Safety - Volume 154, 15 June 2018, Pages 19-26
Journal: Ecotoxicology and Environmental Safety - Volume 154, 15 June 2018, Pages 19-26
نویسندگان
Selma Hurem, Tânia Gomes, Dag A. Brede, Ian Mayer, Viola H. Lobert, Stephen Mutoloki, Kristine B. Gutzkow, Hans-Christian Teien, Deborah Oughton, Peter Aleström, Jan L. Lyche,