کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8865194 | 1620699 | 2018 | 42 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Performance of personal electrostatic bioaerosol sampler (PEBS) when collecting airborne microorganisms
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
علوم زمین و سیارات
علم هواشناسی
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چکیده انگلیسی
We recently developed a new personal electrostatic bioaerosol sampler (PEBS) for determining exposures to airborne microorganisms. PEBS was shown to collect airborne non-biological particles with efficiencies approaching 80% while producing very low ozone concentrations. In this work, we analyzed the performance of this sampler when collecting airborne Bacillus atrophaeus bacterial cells and Penicillium chrysogenum fungal spores as a function of sampling flow rates (e.g., 10 and 20â¯L/min) and sampling time (e.g., 10, 60, and 240â¯min). The collected samples were analyzed using microscopy, adenosine triphosphate (ATP)-based bioluminescence, flow cytometry (Live/Dead test), and culture techniques. PEBS's physical and biological performance was compared against that of BioSampler (SKC Inc., Eighty Four, PA) when the samplers were operated at 10 and 12.5â¯L/min, respectively. PEBS achieved physical collection efficiency as high as 83%, and its physical performance in terms of measured bioaerosol concentration was better than that of BioSampler. In addition, a fraction of live microorganisms recovered by PEBS was not different from that of BioSampler. Compared to BioSampler, PEBS measured similar or higher concentrations of culturable bacteria, but lower concentrations of culturable spores. The airborne ATP concentration measured by PEBS was significantly higher than that measured by BioSampler. Overall, we show that PEBS is a viable and efficient technology to determine personal exposures to airborne microorganisms using multiple sample analysis techniques.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Aerosol Science - Volume 124, October 2018, Pages 54-67
Journal: Journal of Aerosol Science - Volume 124, October 2018, Pages 54-67
نویسندگان
Taewon T. Han, Nirmala M. Thomas, Gediminas Mainelis,