کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8876270 | 1623719 | 2016 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Establishment of PCR-ELISA for Detecting Glyphosate Resistant Transgenic Soybean
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A PCR-ELISA method for detecting the glyphosate resistant transgenic soybean was established and optimized. The results showed that the key parameters of PCR-ELISA were as follows: the concentration of digoxin tag probe was 0.5 µmol · Lâ1, the time of hybridization reaction was 15 min and the chromogenic reaction should last for 30 min. The sensitivity and the repeatability of our PCR-ELISA method were evaluated, and the results showed that it could be detected when the concentration of DNA template from transgenic soybean samples was 0.01% or higher, and the coefficient of variation of this method was less than 5% in our research condition. These results suggested that PCR-ELISA method establishment in this study had good repeatability and high precision for detecting the transgenic soybean samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Northeast Agricultural University (English Edition) - Volume 23, Issue 2, June 2016, Pages 45-51
Journal: Journal of Northeast Agricultural University (English Edition) - Volume 23, Issue 2, June 2016, Pages 45-51
نویسندگان
Yuan Qiang, Wei Yun-min, Fu Ming-ming, Qiu You-wen, Wen Hong-tao, Zhang Ming-hui, Liu Ying, Ao Jin-xia,