کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8879819 | 1624789 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In vitro propagation of Digitalis lanata Ehrh. through direct shoot regeneration - A source of cardiotonic glycosides
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کلمات کلیدی
IAANaAN6-benzyladenineBAPIBADigitoxinTDZPGRsDirect organogenesis - ارگانیسم مستقیمindole-3-butyric acid - اسید Indole-3-Butyricindole-3-acetic acid - اسید Indole-3-استیکα-naphthalene-acetic acid - اسید α-نفتالین-استیکPlant growth regulators - تنظیم کننده های رشد گیاهیDigoxin - دیگوکسینMurashige and Skoog basal medium - محیط موراشیگ و اسگوگ پایهhigh performance liquid chromatography - کروماتوگرافی مایع با کارایی بالاHPLC - کروماتوگرافی مایعی کاراKin - کینkinetin - کینتین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
An efficient method for the in vitro propagation of Digitalis lanata Ehrh. through direct organogenesis from leaf and petiole explants has been standardised. MS basal medium supplemented with cytokinins BAP, KIN, TDZ either alone or along with auxins IAA and NAA at different concentrations were tried. TDZ at 4.54 and 6.81â¯Î¼mol/l were optimum for direct regeneration of shoots from leaf (4.4â¯Â±â¯0.6 shoots/explant), and petiole (3.0â¯Â±â¯0.8 shoots/explant) explants respectively. Among the various concentrations of auxins IAA, IBA and NAA tried for rooting, the best response occurred on MS basal medium supplemented with 17.13â¯Î¼mol/l IAA. On greenhouse transfer about 60% of the plantlets survived. In vitro raised plantlets were morphologically similar to mother plants. Cardiotonic glycosides digoxin and digitoxin were extracted by modified methods and estimated by HPLC. There were no significant differences in digoxin and digitoxin content in leaves of naturally grown and in vitro raised plants. The method for in vitro propagation of D. lanata through direct organogenesis from leaf and petiole explants reported here will be of great use for the rapid and large scale clonal propagation, production of biomass for extraction of cardiotonic glycosides, ex situ conservation, and improvement through conventional plant breeding and transgenic methods.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Industrial Crops and Products - Volume 121, 1 October 2018, Pages 313-319
Journal: Industrial Crops and Products - Volume 121, 1 October 2018, Pages 313-319
نویسندگان
Bhushan P. Bhusare, C.K. John, V.P. Bhatt, T.D. Nikam,