کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8881359 | 1624882 | 2018 | 19 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of proteins contained in aqueous extracts of wheat bran through a proteomic approach
ترجمه فارسی عنوان
شناسایی پروتئین های موجود در عصاره های آب سبوس گندم از طریق یک روش
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کلمات کلیدی
پروتئین سبوس گندم، آلبومین ها، پروتئوم سبوس گندم،
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
چکیده انگلیسی
Proteins of aqueous extracts of wheat bran are usually considered as the albumin fraction, and have been characterized in terms of molecular weight, amino acid content and functional properties. However, there are no reports on the variety of the extracted proteins and their amino acid sequence, despite this information would be useful if the extracts were used in distinct applications. In the present work, the proteins contained in the aqueous extracts of wheat bran were separated by SDS-PAGE. Nineteen bands with molecular weights in the range 8â93â¯kDa were revealed in the SDS-PAGE gels. After tryptic digestion of proteins, the resulting peptides were analyzed by tandem mass spectrometry. Database search against the Viridiplantae subset of the NCBInr protein database resulted in 39 unique proteins, 11 from which had not been previously identified in different sections of the wheat grain through proteomic analysis. Most of the proteins were metabolic and stress defense enzymes, with the storage proteins representing just a minor fraction.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Cereal Science - Volume 80, March 2018, Pages 31-36
Journal: Journal of Cereal Science - Volume 80, March 2018, Pages 31-36
نویسندگان
Guadalupe Chaquilla-Quilca, René Renato Balandrán-Quintana, José Ángel Huerta-Ocampo, Gabriela Ramos-Clamont Montfort, Jesús Guadalupe Luna-Valdez,