کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8883896 | 1625911 | 2018 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Digital gene expression analysis in the gills of Ruditapes philippinarum exposed to short- and long-term exposures of ammonia nitrogen
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کلمات کلیدی
MCDNCBI non-redundant protein sequencesClusters of Orthologous Groups of proteinsBeta tubulinEGF-likeABATUIACycloKOGPfamA2MCyclophilin AABCN-methyl-d-aspartic acidMRPNMDA18S rDNARuditapes philippinarumDGEqRT-PCR18S4-aminobutyrate aminotransferase - 4-آمینوبوتیرات آمینوترانسفرازmalonyl-CoA decarboxylase - malonyl-CoA dearboxylaseAmmonia Nitrogen - آمونیاک نیتروژنactin - اکتین beta actin - بتا آکتینdigital gene expression - بیان ژن دیجیتالTranscriptome - ترانسکریپتومProtein family - خانواده پروتئینToxicity - سمی بودنendoplasmic reticulum - شبکه آندوپلاسمی DEG - شماelongation factor 1 alpha - ضریب اصطکاک 1 آلفاAlpha-2 macroglobulin - ماگروگلوبولین آلفا 2Gene ontology - هستیشناسی ژنیtub - وانquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیmultidrug resistance protein - پروتئین مقاوم در برابر چندین رژیمDifferentially expressed genes - ژن های متفاوت بیان شده استUBI - که در آنATP-binding cassette - کیت اتصال به ATPGlutamine synthetase - گلوتامین سنتتازUbiquitin - یوبیکویتین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Digital gene expression analysis in the gills of Ruditapes philippinarum exposed to short- and long-term exposures of ammonia nitrogen Digital gene expression analysis in the gills of Ruditapes philippinarum exposed to short- and long-term exposures of ammonia nitrogen](/preview/png/8883896.png)
چکیده انگلیسی
Previous study revealed severe toxic effects of ammonia nitrogen on Ruditapes philippinarum including lysosomal instability, disturbed metabolic profiles, gill tissues with damaged structure, and variation of neurotransmitter concentrations. However, the underlying molecular mechanism was not fully understood yet. In the present study, digital gene expression technology (DGE) was applied to globally screen the key genes and pathways involved in the responses to short- and long-term exposures of ammonia nitrogen. Results of DGE analysis indicated that short-term duration of ammonia exposure affected pathways in Dorso-ventral axis formation, Notch signaling, thyroid hormone signaling and protein processing in endoplasmic reticulum. The long-term exposure led to DEGs significantly enriched in gap junction, immunity, signal and hormone transduction, as well as key substance metabolism pathways. Functional research of significantly changed DEGs suggested that the immunity of R. philippinarum was weakened heavily by toxic effects of ammonia nitrogen, as well as neuro-transduction and metabolism of important substances. Taken together, the present study provides a molecular support for the previous results of the detrimental toxicity of ammonia exposure in R. philippinarum, further work will be performed to investigate the specific genes and their certain functions involved in ammonia toxicity to molluscs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquatic Toxicology - Volume 194, January 2018, Pages 121-131
Journal: Aquatic Toxicology - Volume 194, January 2018, Pages 121-131
نویسندگان
Ming Cong, Huifeng Wu, Tengfei Cao, Jiasen Lv, Qing Wang, Chenglong Ji, Chenghua Li, Jianmin Zhao,