کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8886085 1627435 2018 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Loop-mediated isothermal amplification (LAMP) as a confirmatory and rapid DNA detection method for grey seal (Halichoerus grypus) predation on harbour porpoises (Phocoena phocoena)
موضوعات مرتبط
مهندسی و علوم پایه علوم زمین و سیارات اقیانوس شناسی
پیش نمایش صفحه اول مقاله
Loop-mediated isothermal amplification (LAMP) as a confirmatory and rapid DNA detection method for grey seal (Halichoerus grypus) predation on harbour porpoises (Phocoena phocoena)
چکیده انگلیسی
An increasing number of harbour porpoises along the coastlines of northern Europe are victims of attacks by preying grey seals. To confirm grey seal attacks at a molecular level, a fast loop-mediated isothermal amplification (LAMP) method was developed, based on the amplification of the cytochrome b target gene. With this method, saliva residues within bite marks, presumably originating from grey seal attacks, can be detected on-site, using a swab-based method in combination with a portable real-time fluorometer (Genie® II). The developed LAMP assay included an internal amplification control and was validated for the sensitivity, specificity, limit of detection and a spiking experiment was performed with saliva samples from two grey seals. Finally, three stranded harbour porpoises, with injury patterns presumably originating from grey seal attacks, were analysed. Grey seal DNA was successfully determined in a bite mark of one harbour porpoise with the LAMP method. For direct on-site molecular biological investigations without the opportunity to perform more time-consuming and equipment intensive PCR investigations, the developed rapid LAMP assay offers the possibility to confirm that injuries, probably caused by a grey seal, indeed resulted from a grey seal attack.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Sea Research - Volume 140, October 2018, Pages 32-39
نویسندگان
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