کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8892562 | 1628761 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In vitro germination and growth protocols of the ornamental Lophophora williamsii (Lem.) Coult. as a tool for protecting endangered wild populations
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش باغداری
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Lophophora williamsii is an ornamental slow growth cactus highly appreciated by cacti growers and hobbyists. Its demand is often satisfied through illegal collection of wild plants and many populations are threatened with extinction. Thus, an efficient in vitro protocol without plant growth regulators will be of great interest for conservation purposes of this cactus. Eight different germination media, combining Murashige and Skoog medium (MS, full and half-strength), sucrose (20 and 30â¯gâ¯Lâ1) and agar (8 and 10â¯gâ¯Lâ1), were used to study germination rate, number of seedlings with areoles and initial seedling development. Germination rates among culture media only differed significantly in the first 14 days after sowing (DAS), reaching 67-75% at the end of the assay (49 DAS). Remarkable interactions among media components were detected, and 20â¯gâ¯Lâ1 sucrose and 8â¯gâ¯Lâ1 agar combination gave the highest performance for both size and number of areoles. Following germination assay, a growth assay was conducted during 105 days using three growth media (GrM) at different sucrose concentration (15, 30 and 45â¯gâ¯Lâ1) to evaluate the increase in seedling size and number of areoles. Regardless of their initial size, 15â¯gâ¯Lâ1 sucrose provided the best results for both traits. Size increase was higher in the 4-5â¯mm seedling group, while increase in areoles was greater in 2-3â¯mm seedlings. It was possible to develop an in vitro protocol, in absence of plant growth regulators, which allows maximizing L. williamsii germination and growth during its first stages of development, which may increase the availability of plants in the market and avoid exhaustion of wild populations. Furthermore, plants grown ex situ could be reintroduced in endangered natural populations.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Scientia Horticulturae - Volume 237, 14 July 2018, Pages 120-127
Journal: Scientia Horticulturae - Volume 237, 14 July 2018, Pages 120-127
نویسندگان
Cortés-Olmos C., Gurrea-Ysasi G., Prohens J., RodrÃguez-Burruezo A., Fita A.,