کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8920149 | 1643340 | 2014 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Production of transgenic pigs over-expressing the antiviral gene Mx1
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
eGFPIFASCNTIFNs - IFN هاMOI - MEMx1 - MX1indirect immunofluorescence assay - آزمایش ایمنی فلوئورسانس غیر مستقیمSomatic cell nuclear transfer - انتقال هسته سلول سوماتیinterferons - اینترفرون هاInnate resistance - مقاومت درونیplaque-forming unit - واحد پلاک سازیClassical swine fever virus - ویروس کبد خوکenhanced green fluorescence protein - پروتئین فلورسانس سبز افزایش یافته استpfu - پفوmultiplicity of infection - چندین عفونت
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The myxovirus resistance gene (Mx1) has a broad spectrum of antiviral activities. It is therefore an interesting candidate gene to improve disease resistance in farm animals. In this study, we report the use of somatic cell nuclear transfer (SCNT) to produce transgenic pigs over-expressing the Mx1 gene. These transgenic pigs express approximately 15-25 times more Mx1 mRNA than non-transgenic pigs, and the protein level of Mx1 was also markedly enhanced. We challenged fibroblast cells isolated from the ear skin of transgenic and control pigs with influenza A virus and classical swine fever virus (CFSV). Indirect immunofluorescence assay (IFA) revealed a profound decrease of influenza A proliferation in Mx1 transgenic cells. Growth kinetics showed an approximately 10-fold reduction of viral copies in the transgenic cells compared to non-transgenic controls. Additionally, we found that the Mx1 transgenic cells were more resistant to CSFV infection in comparison to non-transgenic cells. These results demonstrate that the Mx1 transgene can protect against viral infection in cells of transgenic pigs and indicate that the Mx1 transgene can be harnessed to develop disease-resistant pigs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Regeneration - Volume 3, Issue 1, 2014, 3:11
Journal: Cell Regeneration - Volume 3, Issue 1, 2014, 3:11
نویسندگان
Quanmei Yan, Huaqiang Yang, Dongshan Yang, Bentian Zhao, Zhen Ouyang, Zhaoming Liu, Nana Fan, Hongsheng Ouyang, Weiwang Gu, Liangxue Lai,