Characterizing various monoclonal antibodies with milder reversed phase chromatography conditions

This work provides examples of milder conditions (65 °C and 0.03% TFA + 0.07% FA) being successfully employed in the RPLC(-MS) analysis of mAb subunits. A suitable stationary phase was selected, and milder conditions allow an improved MS sensitivity, while maintaining comparable elution profiles. Moreover, the use of milder conditions may reduce the risk of artefact peaks.