کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8942676 | 1645111 | 2018 | 29 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Study on a dSPE-LC-MS/MS method for lysophosphatidylcholines and underivatized neurotransmitters in rat brain tissues
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Study on a dSPE-LC-MS/MS method for lysophosphatidylcholines and underivatized neurotransmitters in rat brain tissues Study on a dSPE-LC-MS/MS method for lysophosphatidylcholines and underivatized neurotransmitters in rat brain tissues](/preview/png/8942676.png)
چکیده انگلیسی
A dispersed solid phase extraction method for high performance liquid chromatography-tandem mass spectrometry analysis of lysophosphatidylcholines and underivatized neurotransmitters in rat brain tissues has been developed. Lysophosphatidylcholines (16:0, 18:0, 18:1, 20:4, 22:6), dopamine, 5-hydroxytryptamine, glutamine, dihydroxy-phenyl acetic acid, 5-hydroxyindole acetic acid and γ-aminobutyric acid were included. Dispersed solid phase extraction method was developed with the aiming to minimize the matrix effects. The cleanup procedure has been followed by rat brain homogenate, nitrogen blow drying, reconstitution, dispersive solid phase extraction and separation. Type and size of sorbent, the re-dissolving solution of the tissues, dilute solution, elution solution and the appropriate acidic or alkaline environment of the system were optimized for the purpose of obtaining a minimized matrix effect. Reversed-phase liquid chromatography was used for separation and mass spectrometry with multiple reaction monitoring was used for quantification. Electrospray ionization in negative ion mode was suitable for dihydroxy-phenyl acetic acid and 5-hydroxyindole acetic acid, while the other substances were detected in positive ion mode. The method was validated with linear range, method detection limit, limit of quantitation, matrix effects, relative recovery and the intra and inter-day precision. Linear range was at 0.1-1000â¯ng/mL for γ-aminobutyric acid and glutamine, 1.0-1000â¯ng/mL for dopamine, 10-1000â¯ng/mL for 5-hydroxytryptamine, 50-1000â¯ng/mL for 5-hydroxyindole acetic acid, dihydroxy-phenyl acetic acid and lysophosphatidylcholines. The mean matrix effect was between 52.48% and 123.12% and the CVs ranged from 0.41% to 10.96%. Mean relative recovery was from 65.17% to 114.89%, and the CVs ranged from 0.14% to 8.68% at 3 concentration levels for all compounds. Intra-day precision was with coefficients of variation between 0.20% and 14.7%. Inter-day precision values varied <9.21%, with coefficients of variation between 0.18% and 9.21%. The developed method was used to quantify lysophosphatidylcholines and underivatized neurotransmitters in healthy rat brain tissues in this study.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1096, 1 October 2018, Pages 11-19
Journal: Journal of Chromatography B - Volume 1096, 1 October 2018, Pages 11-19
نویسندگان
Hui Liu, Na Liu, Wenfeng Teng, Jing Chen,