کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8943824 | 1645208 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparison of soy lecithin, coconut water, and coconut milk as substitutes for egg-yolk in semen cryodiluent for black rhinoceros (Diceros bicornis) and Indian rhinoceros (Rhinoceros unicornis)
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
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چکیده انگلیسی
Semen cryopreservation for the black rhinoceros (Diceros bicornis) and Indian rhinoceros (Rhinoceros unicornis) relies on extenders containing egg-yolk (EY). Use of such media is not ideal as inter-batch composition varies and there is risk of pathogenic contamination. The goal of this study was to test animal protein-free extenders. Semen collected via electroejaculation from 10 rhinoceros (6 black, 4 Indian) was diluted with extender containing EY, 1% or 2% soy lecithin (1%SL; 2%SL), coconut water (CW), or coconut milk (CM), cryopreserved and evaluated for sperm motility, viability, morphology, progression, and acrosomal integrity at 0, 1, 3, 6 and 24â¯h post-thaw. Meanâ¯Â±â¯SD fresh ejaculate motility was 84.5â¯Â±â¯7.6%, progression: 3.6â¯Â±â¯0.6 (scale 0-5), viability: 83.4â¯Â±â¯7.1%, intact acrosomes: 71.3â¯Â±â¯6.9%, and morphologically normal: 78.8â¯Â±â¯13.6%. Motility and progression decreased in all groups post-thaw, were greatest in EY, and decreased over time (Pâ¯â¤â¯0.05). Motility and progression did not differ (Pâ¯>â¯0.05) between 1%SL and 2%SL, but were lower (Pâ¯â¤â¯0.05) in CM and CW, and acrosomal integrity was higher (Pâ¯â¤â¯0.05) in EY, 1%SL and 2%SL than in CM and CW. Post-thaw viability was greatest in EY and 2%SL followed by 1%SL, then CM and CW (Pâ¯â¤â¯0.05). Morphology did not differ among treatments (Pâ¯>â¯0.05). Morphology, acrosomal integrity, and viability were maintained over time (Pâ¯>â¯0.05). Although some rhinoceros sperm survived cryopreservation in SL treatments, reduced post-thaw motility rendered all treatments inadequate substitutes for EY-based extenders.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Theriogenology - Volume 121, November 2018, Pages 72-77
Journal: Theriogenology - Volume 121, November 2018, Pages 72-77
نویسندگان
Jessye Wojtusik, Monica A. Stoops, Terri L. Roth,