کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8954797 | 1646046 | 2019 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differentiation of bovine from porcine gelatin capsules using gel electrophoresis method
ترجمه فارسی عنوان
تمایز گاو از کپسول ژلاتین گوشتی با استفاده از روش الکتروفورز ژل
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کلمات کلیدی
ژلاتین، الکتروفورز ژل، حلال، کپسول، بارش سولفات آمونیوم،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Gelatin is commonly used in food supplements and in the form of soft or hard capsules. The source of gelatins is usually from porcine and bovine, and less commonly from vegetable and fish. Nevertheless, these different origins of gelatin have much similarity in term of structures, physicochemical properties and amino acid sequences. Due to these reasons, differentiation of the source of gelatins has been very difficult. In our present study, differentiation of sources of gelatin was made possible in a simplified yet economical method. Sample was prepared using ammonium sulfate precipitation and subjected to gel electrophoresis for protein separation. We have found a fraction of proteins which is able to differentiate porcine and bovine gelatins accurately, with distinctive protein bands in SDS-PAGE at 140â¯kDa and 110â¯kDa for bovine and porcine samples, respectively. This method was verified by 13 double-blinded gelatin samples, all the 13 samples were accurately identified.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 274, 15 February 2019, Pages 16-19
Journal: Food Chemistry - Volume 274, 15 February 2019, Pages 16-19
نویسندگان
Beow Keat Yap, Lay-Harn Gam,