کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8956040 | 1646123 | 2018 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
β-Heregulin impairs EGF induced PLC-γ1 signalling in human breast cancer cells
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کلمات کلیدی
PI3KPLC-γ1PIP2PIP3FCSIP3PLASPCEGFinositol-1,4,5-trisphosphate - inositol-1،4،5-trisphosphateProximity ligation assay - آزمون لمس نزدیکیdiacylglycerol - دیسیل گلیسیرینDAG - روزBreast cancer - سرطان پستانfoetal calf serum - سرم گوساله جنینphosphatidyl-inositol-3-kinase - فسفاتیدیل-انزیتول-3-کینازphospholipase C-γ1 - فسفولیپاز C-γ1Signal transduction - هدایت سیگنالErbB receptors - گیرنده های ErbB
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: β-Heregulin impairs EGF induced PLC-γ1 signalling in human breast cancer cells β-Heregulin impairs EGF induced PLC-γ1 signalling in human breast cancer cells](/preview/png/8956040.png)
چکیده انگلیسی
The interplay of ErbB receptor homo- and heterodimers plays a crucial role in the pathology of breast cancer since activated signal transduction cascades coordinate proliferation, survival and migration of cells. EGF and β-Heregulin are well characterised ligands known to induce ErbB homo- and heterodimerisation, which have been associated with disease progression. In the present study, we investigated the impact of both factors on the migration of MDA-NEO and MDA-HER2 human breast cancer cells. MDA-NEO cells are positive for EGFR and HER3, while MDA-HER2 cells express EGFR, HER2 and HER3. Cell migration analysis revealed that β-Heregulin potently impaired EGF induced migration in both cell lines. Western blot studies showed that both ErbB receptor and PLC-γ1 tyrosine phosphorylation levels were diminished in EGF and β-Heregulin co-treated MDA-NEO and MDA-HER2 cells, which was further correlated to a significantly impaired calcium influx. Our data indicate that EGF and HRG may interfere with each other for receptor binding and dimerisation, which ultimately has an impact on signalling outcome.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Signalling - Volume 52, December 2018, Pages 23-34
Journal: Cellular Signalling - Volume 52, December 2018, Pages 23-34
نویسندگان
Nadine Rommerswinkel, Silvia Keil, Alshaimaa Adawy, Jan G. Hengstler, Bernd Niggemann, Kurt S. Zänker, Thomas Dittmar,