Biodesulfurisation of DBT with Pseudomonas putida CECT5279 by resting cells: Influence of cell growth time on reducing equivalent concentration and HpaC activity

The influence of 4S compound transport across cellular membrane is studied by comparison of results obtained by resting cell assays (whole cells) and with homogenized cells assays (disrupted cells). The results show that there is no accumulation of any compound inside the cells, and that the transport rate across the cellular membrane does not limit the overall biodesulfurisation rate.