کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8978119 | 1106573 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differential expression of FREP genes in two strains of Biomphalaria glabrata following exposure to the digenetic trematodes Schistosoma mansoni and Echinostoma paraensei
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کلمات کلیدی
qPCRTrematodeBiomphalaria glabrataFREPDPE - ECDEchinostoma paraensei - Echinostoma pareneseiQuantitative PCR - PCR کمیDifferential gene expression - بیان ژن دیفرانسیلSchistosoma mansoni - شیستوزومای مانسونیconfidence interval - فاصله اطمینانThreshold value - مقدار آستانهquantitative polymerase chain reaction - واکنش زنجیره ای پلیمراز کمیFibrinogen-related protein - پروتئین مرتبط با فیبرینوژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Fibrinogen-related proteins (FREPs) are hypothesized to function in non-self-recognition in the snail Biomphalaria glabrata. To investigate this assumption, the expression of four members of the FREP gene family was studied using quantitative PCR at 0.5-16 days following exposure of M line and BS-90 strain B. glabrata to Echinostoma paraensei and Schistosoma mansoni. Both strains react to, but fail to eliminate E. paraensei. Only the BS-90 strain is immunologically resistant to S. mansoni. Both snail strains responded to E. paraensei with significantly elevated expression of FREP 2 and 4. Following exposure to S. mansoni, resistant BS-90 snails showed an increase in expression of FREP 2 and 4 (57-fold and 4.5-fold increase, respectively), susceptible M line snails did not display a FREP response. Expression of FREP 3 and 7 was not significantly elevated in any snail/trematode combination. These expression profiles support the hypothesis that some FREPs play a role in the anti-trematode responses in B. glabrata.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 29, Issue 4, 2005, Pages 295-303
Journal: Developmental & Comparative Immunology - Volume 29, Issue 4, 2005, Pages 295-303
نویسندگان
Lynn A. Hertel, Coen M. Adema, Eric S. Loker,