کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8978217 | 1106586 | 2005 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Pathogenesis and inflammatory response to Edwardsiella tarda infection in the zebrafish
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کلمات کلیدی
TSA, tryptic soy agarCFU, colony-forming unit - CFU، واحد تشکیل دهنده کلنیIL-1β, interleukin-1β - IL-1β، اینترلوکین-1βIM, intramuscular - IM، عضلانیIP, intraperitoneal - IP، داخل صفاقیTSB, tryptic soy broth - TSB، سیب زمینی سرخ شدهEdwardsiella tarda - ادواردزیلا تارداTNFα, Tumor Necrosis Factor-α - اکتور نکروز توموری آلفاExperimental infection - عفونت تجربیPBS, phosphate buffered saline - محلول نمکِ فسفات با خاصیت بافریInflammatory response - واکنش التهابیPCR, polymerase chain reaction - واکنش زنجیرهٔ پلیمرازPathogenesis - پاتونزZebrafish - گورخرماهی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The zebrafish (Danio rerio) is a widely used model for developmental biology, neurobiology, toxicology, and genetic disease. Recently, the zebrafish has been recognized as a valuable model for infectious disease and immunity. In this study the pathogenesis and inflammatory cytokine response of zebrafish to experimental Edwardsiella tarda infection was characterized. In challenge experiments, zebrafish embryos were susceptible to infection by immersion. Adult fish were susceptible to challenge by intraperitoneal (ip) injection but not static immersion unless the epithelial layer was perturbed by scraping prior to exposure. To determine if E. tarda infection induces a typical acute inflammatory response, mRNA expression levels of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNFα) were assessed by quantitative real-time PCR. The expression levels of IL-1β and TNFα were significantly upregulated in infected zebrafish embryos and adults. The methods developed in this study will be particularly valuable for targeted gene disruption studies of host immune components and in zebrafish genetic screens.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 29, Issue 6, 2005, Pages 501-513
Journal: Developmental & Comparative Immunology - Volume 29, Issue 6, 2005, Pages 501-513
نویسندگان
Meagan E. Pressley, Peter E. III, P. Eckhard Witten, Mark T. Mellon, Carol H. Kim,