کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8978267 | 1106591 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cloning and characterization of chicken stromal cell derived factor-1
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کلمات کلیدی
SDF-1EST, expressed sequence tag - EST، نشان دهنده توالی بیان شده استRACE, rapid amplification of cDNA ends - RACE، تقویت سریع cDNA به پایان می رسدRT-PCR, Reverse Transcriptase-Polymerase Chain Reaction - RT-PCR، واکنش زنجیره ای ترانسپرتالیست معکوس پلیمریزاسیونGene expression - بیان ژنCalcium flux - شار کلسیمRace - مسابقهChemokines - کرموین ها
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Cloning and characterization of chicken stromal cell derived factor-1 Cloning and characterization of chicken stromal cell derived factor-1](/preview/png/8978267.png)
چکیده انگلیسی
Stromal cell derived factor-1, SDF-1, belongs to the CXC family of chemokines and has been identified in mammals, amphibians, and fish. This chemokine has a diverse array of functions in organogenesis, hematopoeisis, B cell development and recruitment of immune system cells. Here, we report the cloning of the chicken SDF-1 ortholog and examine its temporal and spatial expression. The chicken SDF-1 cDNA contained an open reading frame encoding a predicted protein of 89 amino acids, which shared 40-75% identity to SDF-1 protein in other species. Protein folding simulation predicted a tertiary structure very similar to that obtained for human SDF-1. Recombinant chicken SDF-1 was produced using a prokaryotic expression system and the recombinant protein was shown to be biologically active in a calcium flux assay. The SDF-1 gene was found to be expressed ubiquitously and constitutively in adult tissues and was present as early as the primitive streak stage of chicken embryos.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 29, Issue 2, 2005, Pages 143-152
Journal: Developmental & Comparative Immunology - Volume 29, Issue 2, 2005, Pages 143-152
نویسندگان
L.R. Read, J.A. Cumberbatch, M.M. Buhr, A.J. Bendall, S. Sharif,