کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8980686 1553781 2005 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Immunohistochemical Detection of the p27 Capsid Protein of Caprine Arthritis-Encephalitis Virus (CAEV) in Bone-marrow Cells of Seropositive Goats
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Immunohistochemical Detection of the p27 Capsid Protein of Caprine Arthritis-Encephalitis Virus (CAEV) in Bone-marrow Cells of Seropositive Goats
چکیده انگلیسی
Bone-marrow samples were collected from 48 CAEV-seropositive, symptomless goats (30 kids, 18 adults). The samples were formalin-fixed and processed for histological examination. In addition, all samples were examined immunohistochemically with a monoclonal antibody (1A7) against the p27 capsid protein of maedi-visna virus, an antibody which cross-reacts with the Ca-p27 of CAEV. Samples from 16 goats (10/30 kids, 6/18 adults) showed positive immunolabelling of bone-marrow stromal cells (fibrocytes, endothelial cells and adipocytes) and of scattered macrophages, whereas haematopoietic cells were negative. The detection of viral Ca-p27 protein in bone-marrow fibrocytes was consistent with previous in-vitro studies which indicated that such cells are semi-permissive for CAEV infection. It is speculated that bone-marrow stromal cells represent a viral reservoir in symptomless animals.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Comparative Pathology - Volume 133, Issues 2–3, August–October 2005, Pages 197-200
نویسندگان
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