کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8981173 | 1108143 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of Bovine Somatotropin on Uterine Genes Related to the Prostaglandin Cascade in Lactating Dairy Cows*
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کلمات کلیدی
TBSPPARERαPGHSBovine somatotropin - somatotropin گاوTris-buffered saline - تریس بافر شورUterus - رَحِم یا زِهدانProstaglandin endoperoxide synthase - پروستاگلاندین اندپورکسید سنتازGene - ژن Receptor - گیرنده Estrogen receptor alpha - گیرنده استروژن آلفاperoxisome proliferator-activated receptor - گیرنده فعال فعال پروکسیوم
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
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چکیده انگلیسی
Multiparous Holstein cows, averaging 80 d in milk, were used to examine the effect of exogenous bovine somatotropin (bST) on uterine expression of estrogen receptor α (ERα), prostaglandin endoperoxide synthase-2 (PGHS-2), and peroxisome proliferator-activated receptor δ (PPARδ). About 12 h before expected ovulation in a synchronization protocol, cows were assigned to receive bST (500 mg, n = 11) or serve as untreated controls (n = 10). Cows that ovulated (n = 9 bST, 8 control) were divided within treatment to be killed on d 3 or 7 postovulation. Samples of intercaruncular endometrial tissue from uterine horns ipsilateral to the corpus luteum were collected and stored at â80°C for subsequent mRNA analyses. Endometrial concentrations of ERα and PGHS-2 mRNA transcripts were greater on d 7 than on d 3 of the estrous cycle, but did not differ between treatments. Compared with untreated cows, short-term bST treatment decreased PGHS-2 protein expression at d 7 of the estrous cycle. Concentration of PPARδ mRNA transcript in the uterus decreased between d 3 and 7 of the estrous cycle and was negatively correlated with ERα and PGHS-2 mRNA concentrations. Short-term administration of bST to lactating dairy cows had minimal effects on uterine genes encoding ERα, PGHS-2, and PPARδ at d 3 and 7 of the estrous cycle but there may be an inverse relationship between PPARδ and uterine expression of ERα and PGHS-2 genes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Dairy Science - Volume 88, Issue 2, February 2005, Pages 543-552
Journal: Journal of Dairy Science - Volume 88, Issue 2, February 2005, Pages 543-552
نویسندگان
S.A. Balaguer, R.A. Pershing, C. Rodriguez-Sallaberry, W.W. Thatcher, L. Badinga,