کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8981173 1108143 2005 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of Bovine Somatotropin on Uterine Genes Related to the Prostaglandin Cascade in Lactating Dairy Cows*
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Effects of Bovine Somatotropin on Uterine Genes Related to the Prostaglandin Cascade in Lactating Dairy Cows*
چکیده انگلیسی
Multiparous Holstein cows, averaging 80 d in milk, were used to examine the effect of exogenous bovine somatotropin (bST) on uterine expression of estrogen receptor α (ERα), prostaglandin endoperoxide synthase-2 (PGHS-2), and peroxisome proliferator-activated receptor δ (PPARδ). About 12 h before expected ovulation in a synchronization protocol, cows were assigned to receive bST (500 mg, n = 11) or serve as untreated controls (n = 10). Cows that ovulated (n = 9 bST, 8 control) were divided within treatment to be killed on d 3 or 7 postovulation. Samples of intercaruncular endometrial tissue from uterine horns ipsilateral to the corpus luteum were collected and stored at −80°C for subsequent mRNA analyses. Endometrial concentrations of ERα and PGHS-2 mRNA transcripts were greater on d 7 than on d 3 of the estrous cycle, but did not differ between treatments. Compared with untreated cows, short-term bST treatment decreased PGHS-2 protein expression at d 7 of the estrous cycle. Concentration of PPARδ mRNA transcript in the uterus decreased between d 3 and 7 of the estrous cycle and was negatively correlated with ERα and PGHS-2 mRNA concentrations. Short-term administration of bST to lactating dairy cows had minimal effects on uterine genes encoding ERα, PGHS-2, and PPARδ at d 3 and 7 of the estrous cycle but there may be an inverse relationship between PPARδ and uterine expression of ERα and PGHS-2 genes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Dairy Science - Volume 88, Issue 2, February 2005, Pages 543-552
نویسندگان
, , , , ,