کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8991012 | 1555805 | 2005 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cloning and analysis of a novel cDNA from Trichinella spiralis encoding a protein with an FYVE zinc finger domain
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
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چکیده انگلیسی
A cDNA library from Trichinella spiralis adults 3 days post-infection was screened with a cDNA probe, designated T54, derived from a newborn larvae subtracted cDNA library. Sequence analysis showed that the positive clone contained a cDNA insert of 1464Â bp in length with a single open reading frame of 1290Â bp, which encoded a protein of 429 amino acids with a putative molecular mass of 49.9Â kDa. Database analysis predicted the deduced protein had a leucine zipper motif and an FYVE zinc finger domain. The recombinant fusion protein was expressed and rabbit anti-recombinant protein sera reacted with a single peptide migrating at approximately 55Â kDa in crude worm extract from muscle larvae, adults and newborn larvae stages.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Parasitology - Volume 132, Issues 1â2, 5 September 2005, Pages 27-30
Journal: Veterinary Parasitology - Volume 132, Issues 1â2, 5 September 2005, Pages 27-30
نویسندگان
B.Q. Fu, M.Y. Liu, C.M.O. Kapel, X.P. Meng, Q. Lu, X.P. Wu, Q.J. Chen, P. Boireau,