کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8998215 | 1115610 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Serotonin 5-HT7 receptors coupled to induction of interleukin-6 in human microglial MC-3 cells
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کلمات کلیدی
5-HTIL-6RT-PCR8-OH-DPAT5-methoxytryptamineSB-2699705-carboxamidotryptamine5-MeOT5-CT - 5 CT5-hydroxytryptamine, serotonin - 5-hydroxytryptamine، serotonin8-hydroxy-2-(di-n-propylamino)tetralin - 8-هیدروکسی 2- (دی-نپروپیلامینو) تترالینinterleukin-6 - اینترلوکین ۶Cho - برایChinese Hamster Ovary - تخمدان هامستر چینیReverse transcriptase-polymerase chain reaction - واکنش زنجیره ای واکنش زنجیره ای واکنش زنجیره ای5-HT7 receptor - گیرنده 5-HT7
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب رفتاری
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Brain serotonin 5-HT7 receptors are known to be expressed in neurons and astrocytes. We now report the presence of these receptors in a third type of cell, microglial cells. 5-Hydroxytryptamine (5-HT), 5-carboxamidotryptamine (5-CT), 5-methoxytryptamine (5-MeOT) and 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT) induced concentration-dependent stimulations of cAMP accumulation in the human microglial MC-3 cell line. The maximal effect of 5-HT was 3.4±0.3-fold stimulation (mean±S.E.M., n=5) above basal levels. The rank order of agonist potency (pEC50 values) was 5-CT (7.09)>5-HT (6.13)â¥5-MeOT (5.78)â«8-OH-DPAT (ca. 5). The effect of 5-CT was inhibited in a concentration-dependent manner by the selective 5-HT7 receptor antagonist SB-269970 (pA2 value 9.03). Western blot analysis revealed the presence of immunoreactive bands corresponding to the human 5-HT7 receptor in extracts of MC-3 cells. The presence of two splice variants of the 5-HT7 receptor (5-HT7(a/b)) was visualized by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis with specific primers. In real-time PCR studies, the mRNA for interleukin-6 (IL-6) was found to be increased by 2.5-fold in MC-3 cells after 1 h incubation with 5-CT (1 μM) and this effect was fully blocked by the 5-HT7 receptor antagonist SB-269970 (1 μM). These data show that functional 5-HT7 receptors are present in human microglial MC-3 cells, suggesting that they are involved in neuroinflammatory processes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuropharmacology - Volume 49, Issue 1, July 2005, Pages 40-47
Journal: Neuropharmacology - Volume 49, Issue 1, July 2005, Pages 40-47
نویسندگان
Cécile Mahé, Erika Loetscher, Kumlesh K. Dev, Ionel Bobirnac, Uwe Otten, Philippe Schoeffter,