کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9007947 | 1122726 | 2005 | 16 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Detection of a novel population of fetal thymocytes characterized by preferential emigration and a TCRγδ+ T cell fate after dioxin exposure
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کلمات کلیدی
AHRTCDDDRECD4+CD8+ double positiveFTOCCD4−CD8− double negative2,3,7,8-Tetrachlorodibenzo-p-dioxin - 2،3،7،8-تترا کلریدیبنزوپتوفان دیوکسینLineage commitment - تعهد خطیsingle positive - تک مثبتThymus - تیموسDioxin-responsive element - عنصر پاسخ دهنده دیوکسینfetal thymus organ culture - فرهنگ ارگانیزم جنین تیزوسEmigration - مهاجرتarylhydrocarbon receptor - گیرنده arylhydrocarbonaryl hydrocarbon receptor - گیرنده آرویل هیدروکربن
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Detection of a novel population of fetal thymocytes characterized by preferential emigration and a TCRγδ+ T cell fate after dioxin exposure Detection of a novel population of fetal thymocytes characterized by preferential emigration and a TCRγδ+ T cell fate after dioxin exposure](/preview/png/9007947.png)
چکیده انگلیسی
T cell maturation into TCRαβ+ or TCRγδ+ cells from common immature CD4âCD8â(DN) precursors occurs in the thymus, and is controlled through ordered regulation of genes. The aryl hydrocarbon receptor (AHR), a latent cytoplasmic transcription factor, affects thymocyte maturation and differentiation at several stages, also including DN cells. We analyzed in murine fetal thymus organ cultures (FTOC) the outcome of AHR-signaling and found a higher frequency of DN TCRγδ+ cells in the presence of the AHR-activating ligand TCDD. We detected a novel population of CD25int/loCD44hi cells associated with preferential emigration and a TCRγδ+ T cell fate of thymocytes. Sorted DN TCRγδ+ emigrants could proliferate if IL-2 was available. Moreover, they suppressed the proliferation of co-cultivated, activated CD4+ T cells. Gene expression profiles of purified DN emigrants from TCDD*FTOC revealed 295 modulated genes, 10% of which are genes of the immune system. For instance, RAG-1, TdT, and Gfi-1 were downregulated, yet genes indicative of mature thymocytes were upregulated. In conclusion, we have detected changes in the differentiation programme of fetal DN thymocytes after ligand-activation of the AHR. In particular, we observed a higher frequency of DN TCRγδ+ cells with high emigration potential, and possible regulatory functions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Immunopharmacology - Volume 5, Issue 12, November 2005, Pages 1659-1674
Journal: International Immunopharmacology - Volume 5, Issue 12, November 2005, Pages 1659-1674
نویسندگان
Marc Majora, Markus Frericks, Vladimir Temchura, Gaby Reichmann, Charlotte Esser,