کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9012305 | 1124554 | 2005 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A reverse-phase HPLC and fluorescence detection method for measurement of 5-hydroxytryptamine (serotonin) in Planaria
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
داروشناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A reverse-phase HPLC and fluorescence detection method for measurement of 5-hydroxytryptamine (serotonin) in Planaria A reverse-phase HPLC and fluorescence detection method for measurement of 5-hydroxytryptamine (serotonin) in Planaria](/preview/png/9012305.png)
چکیده انگلیسی
Introduction:Planaria have proven to be a good model system in which to investigate mammalian behaviors and responses to drugs. We have recently studied the response of planarians to dopaminergic ligands and to the effects of cocaine and opioids. To correlate behavior (specifically, drug withdrawal) with neurotransmitter levels, we developed a method to quantify 5-hydroxytryptamine (5-HT; serotonin) in planarians. Methods: Following the homogenization of planarians in aqueous solvent (perchloric acid, l-cystine, and Na2EDTA) and centrifugation of supernatant (14,000Ãg at 4 °C for 20 min), 5-HT was measured using HPLC (aqueous citric acid buffer mobile phase; 5-μm C18 column with fluorescence detection, 280/340 nm). NÏ-methyl-5-HT was used as an internal standard (IS). Results: 5-HT was rapidly extracted and conveniently measured from the planarians. The detection limit of the procedure (0.35 ng) was below the amount of 5-HT in one animal. Discussion: The ability to measure neurotransmitter levels provides a methodological opportunity to correlate behavior with biochemical changes in planarians and to extend behavioral observations to intracellular transmitter and second messenger transduction pathways.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmacological and Toxicological Methods - Volume 51, Issue 1, JanuaryâFebruary 2005, Pages 73-76
Journal: Journal of Pharmacological and Toxicological Methods - Volume 51, Issue 1, JanuaryâFebruary 2005, Pages 73-76
نویسندگان
Sumiyo Umeda, Gregory W. Stagliano, Michael R. Borenstein, Robert B. Raffa,