کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9017731 | 1128665 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In vitro assessment of sensitizing activity of low molecular weight compounds
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Predictive tests to identify the sensitizing properties of chemicals are carried out using animals. There is as yet, no accepted in vitro method for the identification of skin sensitizing chemicals. Such in vitro tests should encompass (parts of) the sensitization phase of contact hypersensitivity. Two cell types are predominantly involved in this process, keratinocytes (KC) and Langerhans cells, the latter being a specialized type of skin dendritic cells (DC). Low molecular weight chemicals act as haptens; KC respond to allergen contact by, among others, producing proinflammatory cytokines, while DC take up the haptenized protein, migrate, and present antigen to T-cells. During migration, DC mature, resulting in a loss of antigen uptake capacity, thereby increasing expression of certain surface molecules. Thus, both cytokine production by KC and surface marker expression by DC may be used as in vitro models for the identification of sensitizers. Several reports have shown that intracellular IL-1α is a promising candidate to identify sensitizers using KC. We have recently shown that the potency of sensitizers may be determined by dose-response analysis of intracellular IL-1α and IL-18 using a murine KC cell line. The ranking of potency using this in vitro method was similar to the ranking previously established using the local lymph node assay. Using DC, effects on the expression of various cell surface markers, cytokines, and molecules involved in antigen uptake have been shown to identify sensitizers. One study showed that also the potency of sensitizers may be determined using DC. Additional studies are required to establish whether KC or DC, or combinations thereof, are most suitable for in vitro identification and potency assessment of sensitizers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology and Applied Pharmacology - Volume 207, Issue 2, Supplement, 1 September 2005, Pages 142-148
Journal: Toxicology and Applied Pharmacology - Volume 207, Issue 2, Supplement, 1 September 2005, Pages 142-148
نویسندگان
Rob J. Vandebriel, François M.M. Van Och, Henk van Loveren,