کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9126953 1569967 2005 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Alternative mRNA splice forms of NOXO1: Differential tissue expression and regulation of Nox1 and Nox3
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Alternative mRNA splice forms of NOXO1: Differential tissue expression and regulation of Nox1 and Nox3
چکیده انگلیسی
The activity of gp91phox, the catalytic subunit of the superoxide-generating respiratory burst oxidase, is stimulated by the regulatory subunits p47phox, p67phox and the small GTPase Rac. Novel homologs of p47phox and p67phox (NOXO1 and NOXA1, respectively) were recently identified and are implicated in the regulation of the gp91phox homologs Nox1 and Nox3. Herein, we report four splice forms of human NOXO1. NOXO1β is the major mRNA splice form in human colon and fetal liver while NOXO1γ was the majority species in testis. Neither the α nor δ forms were expressed in significant amounts in any tissue tested. Splice forms were generated by alternative splicing of the two ends of exon 3 of the NOXO1 gene, and resulted in differences in the PX domain. The PX domain is known to bind inositol lipids, but the expressed, purified PX domains from NOXO1β and NOXO1γ bound these lipids with the same specificity and affinity. NOXO1β and NOXO1γ both activated Nox1, but NOXO1γ showed a poorer ability to activate Nox3 compared with NOXO1β. These data suggest different tissue localizations and functions for NOXO1β and NOXO1γ in regulating Nox family members.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 356, 15 August 2005, Pages 118-126
نویسندگان
, ,