کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9127054 | 1160197 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of the origin recognition complex (ORC) from a higher plant, rice (Oryza sativa L.)
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کلمات کلیدی
CDKORCCDCACSARSSAMOryza sativaMCMRPAreplication protein A - تلقیح پروتئین AAutonomous replication sequence - توالی تکاملی مستقلCell proliferation - تکثیر سلولیminichromosome maintenance - نگهداری مینیکروموزومDNA replication - همانندسازی DNA، تکثیر DNApre-RC - پیش RCpre-replication complex - پیچیده قبل از تکثیرorigin recognition complex - پیچیدگی شناخت اولیهCell division cycle - چرخه تقسیم سلولیcyclin-dependent kinase - کییناز وابسته به سیکلینPlant - گیاه
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The origin recognition complex (ORC) protein plays a critical role in DNA replication through binding to sites (origins) where replication commences. The protein is composed of six subunits (ORC1 to 6) in animals and yeasts. Our knowledge of the ORC protein in plants is, however, much less complete. We have performed cDNA cloning and characterization of ORC subunits in rice (Oryza sativa L. cv. Nipponbare) in order to facilitate study of plant DNA replication mechanisms. Our previous report provided a description of a gene, ORC1 (OsORC1), that encodes one of the protein subunits. The present report extends this initial analysis to include the genes that encode four other rice ORC subunits, OsORC2, 3, 4 and 5. Northern hybridization analyses demonstrated the presence of abundant transcripts for all OsORC subunits in shoot apical meristems (SAM) and cultured cells, but not in mature leaves. Interestingly, only OsORC5 showed high levels of expression in organs in which cell proliferation is not active, such as flag leaves, the ears and the non-tip roots. The pattern of expression of OsORC2 also differed from other OsORC subunits. When cell proliferation was temporarily halted for 6-10 days by removal of sucrose from the growth medium, expression of OsORC1, OsORC3, OsORC4 and OsORC5 was substantially reduced. However, the level of expression of OsORC2 remained constant. We suggest from these results that expression of OsORC1, 3, 4 and 5 are correlated with cell proliferation, but the expression of OsORC2 is not.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 353, Issue 1, 20 June 2005, Pages 23-30
Journal: Gene - Volume 353, Issue 1, 20 June 2005, Pages 23-30
نویسندگان
Yoko Mori, Taichi Yamamoto, Norihiro Sakaguchi, Toyotaka Ishibashi, Tomoyuki Furukawa, Yasuhiro Kadota, Kazuyuki Kuchitsu, Junji Hashimoto, Seisuke Kimura, Kengo Sakaguchi,