کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9140096 | 1163396 | 2005 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular cloning and characterization of Ras- and Raf-homologues from the fox-tapeworm Echinococcus multilocularis
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کلمات کلیدی
EGFRASRBDRAFCRDGEFGTPase Activating Protein - GTPase فعال کردن پروتئینMitogen activated protein - Mitogen فعال پروتئینMAP kinase cascade - آبشار کیناز MAPParasite - انگلEchinococcus - اکینوکوکrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدbase pairs - جفت پایهRAS-binding domain - دامنه اتصال RAScysteine-rich domain - دامنه غنی از سیستینSignaling - سیگنالینگGAP - شکافepidermal growth factor - عامل رشد اپیدرمیguanine nucleotide exchange factor - فاکتور تبادل نوکلئوتید گوانینRace - مسابقهconserved region - منطقه حفاظت شدهmap - نقشه
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In addition, we fully characterized the gene emraf whose product, EmRaf, displayed considerable homology to mammalian Raf-kinases and orthologous factors from Drosophila and Caenorhabditis elegans. emraf was co-expressed with emras in the larval stages metacestode and protoscolex during in vitro cultivation and during an infection of the intermediate host as assessed by RT-PCR experiments. The emraf gene was composed of nine exons and eight introns and shared four highly conserved exon-intron boundaries with the human gene encoding Raf-1, suggesting that both genes derived from a common evolutionary ancestor. Southern blot hybridizations demonstrated that emraf is a single copy gene. Using the yeast two-hybrid system, EmRaf was shown to interact with EmRas, but not with EmRal, a previously characterized orthologue of mammalian Ral GTPases. This is the first characterization of a Ras orthologue from a cestode and the first report on a Raf-like kinase from a platyhelminth. The data presented herein will form a solid basis for further investigations on Echinococcus signaling systems that are involved in growth control and development of the parasite.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Biochemical Parasitology - Volume 139, Issue 2, February 2005, Pages 225-237
Journal: Molecular and Biochemical Parasitology - Volume 139, Issue 2, February 2005, Pages 225-237
نویسندگان
Markus Spiliotis, Dennis Tappe, Stefan Brückner, Hans-Ulrich Mösch, Klaus Brehm,