کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9140152 | 1163399 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Insights into a CRM1-mediated RNA-nuclear export pathway in Trypanosoma cruzi
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کلمات کلیدی
U snRNAspliced leader RNAUBPLMBPABP1Crm1nESNLSNPCUTRCCRSL RNA - RNA SLTrypanosoma - تریپانوزوما، مته تنnuclear export signal - سیگنال صادرات هسته ایnuclear localization signal - سیگنال محلی سازی هسته ایAU-rich element - عنصر غنی AULeptomycin B - لپتومایسین Buntranslated region - منطقه غیر ترجمهARE - هستند
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Insights into a CRM1-mediated RNA-nuclear export pathway in Trypanosoma cruzi Insights into a CRM1-mediated RNA-nuclear export pathway in Trypanosoma cruzi](/preview/png/9140152.png)
چکیده انگلیسی
Nuclear export and import of proteins and RNAs is a regulated process that permits the control of protein expression during cell development and differentiation. In all eukaryotic organisms transport of proteins to specific cellular compartments requires specific signaling sequences. Proteins that shuttle between nucleus and cytoplasm bear nuclear localization signals (NLS) and/or nuclear export signals (NES) and some of them can carry mRNAs, as part of shuttling ribonucleoprotein complexes. In this work we describe in the protozoan parasite Trypanosoma cruzi, a CRM1/exportin1 nuclear export factor named TcCRM1. This protein contains the conserved central region (CCR) that interacts with NES sequences present within cargo molecules, and the Cys residue involved in covalent binding to the Streptomyces metabolite leptomycin B (LMB). By subcellular fractionation we show that TcCRM1, a protein of about 117Â kDa, has nuclear localization. We also demonstrate that LMB inhibits the replication of T. cruzi in a dose-dependent manner. In situ hybridization experiments performed with a Texas red-coupled oligo(dT) probe revealed that LMB produced a partial short-term accumulation of a poly(A)Â +Â RNA subset in the nucleus. Some mRNAs such as HSP70, TcUBP2/1 and TcPABP1 are reduced or disappeared from the cytoplasm of LMB treated cells. In sharp contrast with metazoans, no effect was observed on two U snRNAs subcellular localization, implying that a different export route might exist for these RNAs in trypanosomes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Biochemical Parasitology - Volume 139, Issue 1, January 2005, Pages 15-24
Journal: Molecular and Biochemical Parasitology - Volume 139, Issue 1, January 2005, Pages 15-24
نویسندگان
Ileana C. Cuevas, Alberto C.C. Frasch, Iván D'Orso,