کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9144667 | 1164530 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Two novel Blumeria graminis f. sp. hordei genes are induced in planta and up-regulated in mlo virulent isolates
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کلمات کلیدی
GPDkilo base pair(s)UTRBlumeria graminis f. sp. hordeiRFLPsBGHHAIRT-PCRgDNAORFgenomic DNA - DNA ژنومیReal-time PCR - PCR به موقعsemi-quantitative RT-PCR - RT-PCR نیمه کمیBIG - بزرگGene expression - بیان ژنBase pair(s) - جفت پایه (ها)Barley - جو Susceptibility - حساسیتHordeum vulgare - ذرتhours after inoculation - ساعت ها بعد از تلقیحDifferential display - صفحه نمایش دیفرانسیلopen reading frame - قاب خواندن بازuntranslated region - منطقه غیر ترجمهIsoelectric point - نقطه ایزوالکتریکreverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی-پلیمراز معکوسrestriction fragment length polymorphisms - پلی مورفیسم طول قطعه قطعهPowdery mildew - کپک پودریglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The differential display technique was applied to the Blumeria graminis f. sp. hordei (Bgh)-barley interaction to identify fungal genes involved in the early stages of infection. Out of 33 cDNA fragments originally isolated, nine were confirmed by RT-PCR to represent differentially expressed Bgh genes. cDNA and genomic clones of three novel genes, BIG1, BIG2, and BIG4 (Barley-induced gene 1, 2, and 4), were characterized. They are predicted to encode secreted proteins and to be specific for the Bgh-barley interaction. In the Bgh isolate Race I, an mlo virulent isolate, they are highly up-regulated during penetration and primary haustorium formation. Expression of the BIG1 and BIG2 genes was further analysed in two near-isogenic Bgh isolates, one mlo virulent, HL3/5, and one avirulent, GE3, during the interaction with a susceptible cultivar. In both isolates the genes are up-regulated at the appressorial stage and in HL3/5 expression increases further during penetration and primary haustorium formation. This was also observed in Race I, indicating a different regulation of BIG1 and BIG2 in mlo virulent isolates.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Physiological and Molecular Plant Pathology - Volume 66, Issue 3, March 2005, Pages 79-89
Journal: Physiological and Molecular Plant Pathology - Volume 66, Issue 3, March 2005, Pages 79-89
نویسندگان
Morten N. Grell, Kirsten B. Holm, Henriette Giese,