کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9185024 | 1183032 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of TNF-α and curcumin on the expression of thrombomodulin and endothelial protein C receptor in human endothelial cells
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کلمات کلیدی
tPAEPCRIL-8APCTAFIEBM-2ICAM-1HUVECSPAI-1HMECsHCAECsIL-1Antioxidant - آنتی اکسیدانinflammation - التهاب( توروم) Interleukin-8 - اینترلوکین -8interleukin-1 - اینترلوکین-1Thrombomodulin - ترومبومودولینtumor necrosis factor-alpha - تومور نکروز عامل آلفاhuman coronary artery endothelial cells - سلول اندوتلیال عروق کرونر انسانیEndothelial cell - سلول های اندوتلیالHuman umbilical vein endothelial cells - سلول های اندوتلیالی ورید ناف انسانhuman dermal microvascular endothelial cells - سلولهای اندوتلیال میکرواسکولی پوست انسانHuman lung microvascular endothelial cells - سلولهای اندوتلیوم میکروویو انسانی ریه انسانTissue factor - عامل بافتVascular endothelial growth factor - فاکتور رشد اندوتلیال عروقیVascular Endothelial Growth Factor (VEGF) - فاکتور رشد اندوتلیال عروقی (VEGF)Human basic fibroblast growth factor - فاکتور رشد فیبروبلاست اساسی انسانTNF-α - فاکتور نکروز توموری آلفاTissue-type plasminogen activator - فعال کننده بافت پلاسمینوژنendothelial cell basal medium - محیط پایه سلول اندوتلیالThrombin activatable fibrinolysis inhibitor - مهار کننده فیبرینولیز فعال فعال ترومبینintercellular adhesion molecule-1 - مولکول چسبندگی بین سلولی -1Activated protein C - پروتئین فعال Cplasminogen activator inhibitor type-1 - پلاسمینوژن فعال کننده مهار کننده نوع 1threshold cycle - چرخه آستانهCurcumin - کورکومینendothelial protein C receptor - گیرنده پروتئین C اندوتلیال
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
کاردیولوژی و پزشکی قلب و عروق
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The objective of this study was to elucidate the effects of tumor necrosis factor-alpha (TNF-α) on the expression of thrombomodulin (TM) and endothelial protein C receptor (EPCR) in human endothelial cells as well as the effect of curcumin, a spice and coloring food compound, as a potential therapeutic agent. Human umbilical vein endothelial cells (HUVECs) treated with TNF-α (2.0 ng/ml) showed reduced TM mRNA levels by 80%, 97%, 94%, and 97% at 3, 6, 12, and 24 h, respectively (P<0.05), by real-time PCR analysis. Dose-dependent study showed that TM mRNA levels of HUVECs were decreased by 86%, 89%, 91%, and 94% after treatment of TNF-α (0, 0.25, 0.5, 1, and 2 ng/ml) for 6 h, respectively (P<0.05). TM protein levels in HUVECs were significantly reduced by 69% in TNF-α-treated cells as compared to controls (P<0.05) by Western blot analysis. Secreted protein and activity of TM of HUVEC cultures were also significantly reduced in TNF-α-treated cells. In addition, EPCR mRNA levels of HUVECs were significantly reduced in TNF-α-treated group as compared to controls (P<0.05). Furthermore, these effects were observed in other types of endothelial cells from human coronary arteries, lung, and skin. Curcumin effectively blocked these effects of TNF-α on downregulation of TM and EPCR. These data demonstrate that TNF-α significantly decreases expression of TM and EPCR at both mRNA and protein levels in several human endothelial cells. Curcumin can effectively block TNF-α-induced endothelial dysfunction. This study suggests a new molecular mechanism of inflammation-induced thrombosis and a new therapeutic strategy to prevent this clinical problem.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Thrombosis Research - Volume 115, Issue 5, 2005, Pages 417-426
Journal: Thrombosis Research - Volume 115, Issue 5, 2005, Pages 417-426
نویسندگان
Bicheng Nan, Peter Lin, Alan B. Lumsden, Qizhi Yao, Changyi Chen,