کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9185484 | 1183190 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Iron-mediated stability of PAI-1 mRNA in adenocarcinoma cells-involvement of a mRNA-binding nuclear protein
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کلمات کلیدی
HEPESDRBPAI-1mRNA binding protein3′-UTR - 3'-UTR3′-untranslated region - 3'-ناحیه ترجمه نشدهmRNA stability - ثبات mRNADigoxygenin - دیگوکسینژینDIG - شماIron deprivation - محرومیت آهنPost-transcriptional regulation - مقررات پست مدرنType-1 plasminogen activator inhibitor - مهار کننده فعال کننده پلاسمینوژن نوع 1
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
کاردیولوژی و پزشکی قلب و عروق
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
This study reports the stability of mRNA of type-1 plasminogen activator inhibitor (PAI-1), the major physiologic inhibitor of plasminogen activation, by deferoxamine-aided iron deprivation, in PC3 adenocarcinoma cells. ELISA and Northern analyses studies revealed dose-dependent increase in PAI-1 expression by deferoxamine-treated cells. Co-treatment with ferric citrate quenched the effect of deferoxamine, confirming the role of iron in PAI-1 regulation. DRB-based RNA chase experiments suggested that post-transcriptional mechanism was involved in PAI-1 regulation. De-novo protein synthesis was necessary for this regulation. Electrophoretic mobility shift assay revealed the presence of a nuclear protein, binding to the 3â²-UTR of PAI-1 mRNA in an iron-mediated manner. This is the first report of iron-mediated mRNA-protein interaction in PAI-1, involved in mRNA stability.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Thrombosis Research - Volume 116, Issue 3, 2005, Pages 255-263
Journal: Thrombosis Research - Volume 116, Issue 3, 2005, Pages 255-263
نویسندگان
K.S. Radha, Masahiko Sugiki, Etsuo Yoshida, M. Harish Kumar, Sayuri Omura, Masugi Maruyama,