کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9194637 | 1580510 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of microglia induced from mouse embryonic stem cells and their migration into the brain parenchyma
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
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چکیده انگلیسی
We derived microglia from mouse embryonic stem cells (ES cells) at very high density. Using the markers Mac1+/CD45low and Mac1+/CD45high to define microglia and macrophages, respectively, we show that Mac1+ cells are induced by GM-CSF stimulation following neuronal differentiation of mouse ES cells using a five-step method. CD45low expression was high and CD45high expression was low on induced cells. We used a density gradient method to obtain a large amount of microglia-like cells, approximately 90% of Mac1+ cells. Microglia-like cells expressed MHC class I, class II, CD40, CD80, CD86, and IFN-γR. The expression level of these molecules on microglia-like cells was barely enhanced by IFN-γ. Intravenously transferred GFP+ microglia derived from GFP+ ES cells selectively accumulated in brain but not in peripheral tissues such as spleen and lymph node. GFP+ cells were detected mainly in corpus callosum and hippocampus but were rarely seen in cerebral cortex, where Iba1, another marker of microglia, is primarily expressed. Furthermore, both GFP+ and Iba1+ cells exhibited a ramified morphology characteristic of mature microglia. These studies suggest that ES cell-derived microglia-like cells obtained using our protocol are functional and migrate selectively into the brain but not into peripheral tissues after intravenous transplantation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Neuroimmunology - Volume 160, Issues 1â2, March 2005, Pages 210-218
Journal: Journal of Neuroimmunology - Volume 160, Issues 1â2, March 2005, Pages 210-218
نویسندگان
Takahiro Tsuchiya, Kae Chang Park, Shinichi Toyonaga, Shoko M. Yamada, Hiromichi Nakabayashi, Eiichi Nakai, Naoki Ikawa, Masato Furuya, Akira Tominaga, Keiji Shimizu,