کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9194753 | 1580511 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Immunohistochemical detection of the human cytochrome P4507B1: production of a monoclonal antibody after cDNA immunization
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
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چکیده انگلیسی
The cytochrome P4507B1 (P4507B1) is responsible for the 7α-hydroxylation of dehydroepiandrosterone (DHEA) and other 3β-hydroxysteroids in the brain and other organs. The cDNA of human P4507B1 was used for DNA immunization of mice. The best responding mouse led to the production of monoclonal antibodies (mAbs). The clone D16-37 produced an IgM specific for P4507B1 with no cross-reaction with other human P450s. This antibody permitted the immunohistochemical detection of P4507B1 in slices of human hippocampus. P4507B1 was expressed in neurons only. This new tool will be used for the extensive examination of the P4507B1 presence and determination of its levels in slices of human normal and diseased brain and in other human tissues.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Neuroimmunology - Volume 159, Issues 1â2, February 2005, Pages 41-47
Journal: Journal of Neuroimmunology - Volume 159, Issues 1â2, February 2005, Pages 41-47
نویسندگان
Catherine Trap, Farida Nato, Sonia Chalbot, Sae-Bom Kim, Pierre Lafaye, Robert Morfin,