Protective effect of trapidil against oxidative organ damage in burn injury

Thirty Wistar rats were randomly divided into five groups. Sham group (n = 6) was exposed to 21 °C water while burn-3 h group (n = 6) and burn + trap-3 h group (n = 6), burn-24 h (n = 6) and burn + trap-24 h groups were exposed to boiling water for 12 s to produce a full thickness burn in 35-40% of total body surface area. In both burn + trap-3 h and burn-trap-24 h group, 8 mg/kg trapidil was given intravenously immediately after thermal injury. Three and 24 h later, tissue samples were taken for biochemical analysis from lung, intestine and kidney and blood samples were obtained to determinate serum TNF-α levels. Cutaneous thermal injury caused a significant increase in myeloperoxidase (MPO) activity and malondialdehyde (MDA) and 3-nitrotyrozine (3-NT) levels in all tissues and elevated serum TNF-α levels at post-burn 3 and 24 h. Trapidil treatment significantly reduced in biochemical parameters, as well as serum TNF-α levels. These data suggest that trapidil has a protective effect against oxidative organ damage in burn injury.