کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9244143 | 1209903 | 2005 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Interleukin-13 Is the Key Effector Th2 Cytokine in Ulcerative Colitis That Affects Epithelial Tight Junctions, Apoptosis, and Cell Restitution
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کلمات کلیدی
FACSTNFLPMCEGTANF-κBethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid - اتیلن گلیکول بیس (β-آمینویل اتر) -N، N، N '، N'-tetraacetic اسیدinterferon - اینترفرونIFN - اینترفرون هاinterleukin - اینترلوکینterminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling - برچسب ترمودینامیک deoxyuridine triphosphate ترمینال deoxynucleotidyl transferase ترمینالTUNEL - تونلNKT cell - سلول NKTnatural killer T cell - سلول T قاتل طبیعیlamina propria mononuclear cell - سلول تک هسته ای لامین پروپریاtumor necrosis factor - فاکتور نکروز تومورnuclear factor κB - فاکتور هسته ای κBlactate dehydrogenase - لاکتات دهیدروژناز LDH - لاکتات دهیدروژناز به صورت مختصر شده LDH transepithelial electrical resistance - مقاومت الکتریکی transepithelialpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمراز
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
بیماریهای گوارشی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Background & Aims: Ulcerative colitis (UC) is characterized by a Th2 immune response with inflammation and epithelial barrier dysfunction. So far, Th2 cytokines have not been shown to directly influence epithelial barrier function. Methods: Lamina propria mononuclear cells (LPMCs) were stimulated and interleukin (IL)-13 was measured by enzyme-linked immunosorbent assay. Functional IL-13 and IL-4 effects were studied on HT-29/B6 colonic epithelial cells in Ussing chambers and by conductance scanning. Apoptosis was detected by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling assays. IL-13/IL-4 receptors were analyzed by reverse-transcription polymerase chain reaction and immunofluorescence. Western blotting combined with immunofluorescence was used to detect tight junction proteins. Furthermore, restitution velocity was measured. Finally, mucosal biopsy specimens from patients with UC were compared with cultured cells for these features. Results: LPMCs from patients with UC produced large amounts of IL-13 (985 ± 73 pg/mL), much more than from controls or patients with Crohn's disease. IL-13Rα1 and IL-4Rα receptors were present in HT-29/B6 cells and colonic epithelial cells of control patients and patients with UC. IL-13 had a dose-dependent effect on transepithelial resistance of HT-29/B6 monolayers (reduction to 60% ± 4%), whereas IL-4 had no effect. This was due to an increased number of apoptotic cells (5.6-fold ± 0.9-fold) and an increased expression of the pore-forming tight junction protein claudin-2 to 295% ± 37%, both of which contributed equally. Finally, epithelial restitution velocity decreased from 15.1 ± 0.6 to 10.6 ± 0.5 μm/h after treatment with IL-13. Parallel changes were observed in human samples, with an increase in claudin-2 expression to 956% ± 252%. Conclusions: IL-13 was identified as an important effector cytokine in UC that impairs epithelial barrier function by affecting epithelial apoptosis, tight junctions, and restitution velocity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gastroenterology - Volume 129, Issue 2, August 2005, Pages 550-564
Journal: Gastroenterology - Volume 129, Issue 2, August 2005, Pages 550-564
نویسندگان
Frank Heller, Peter Florian, Christian Bojarski, Jan Richter, Melanie Christ, Bernd Hillenbrand, Joachim Mankertz, Alfred H. Gitter, Nataly Bürgel, Michael Fromm, Martin Zeitz, Ivan Fuss, Warren Strober, Jörg D. Schulzke,