کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9278927 1223033 2005 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Carbon source dependent phosphorylation of the Gpr1 protein in the yeast Yarrowia lipolytica
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Carbon source dependent phosphorylation of the Gpr1 protein in the yeast Yarrowia lipolytica
چکیده انگلیسی
The Gpr1 protein of the ascomycetous yeast Yarrowia lipolytica belongs to the poorly characterised Gpr1/Fun34/YaaH protein family whose members have been only found in prokaryotes and lower eukaryotes so far. Gpr1p seems to be involved in acetic acid adaptation at low pH values. Here we show that Gpr1p is subjected to phosphorylation in dependence on the carbon source. Exhaustion of the carbon source resulted in a complete dephosphorylation of Gpr1p, whereas addition of a new carbon source caused the phosphorylation of Gpr1p. Almost all Gpr1p molecules became phosphorylated after addition of acetate, while other carbon sources only triggered the phosphorylation of about half of the Gpr1p molecules. Phosphorylation was found to occur at serine-37. In spite of the clear effect of acetate/acetic acid on the level of phosphorylation of Gpr1p, no correlation of phosphorylation/dephosphorylation and acetic acid hypersensitivity, caused by mutations within Gpr1p, was detected.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEMS Yeast Research - Volume 5, Issue 10, July 2005, Pages 909-917
نویسندگان
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