کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9296487 | 1233535 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Oligonucleotide-microarray analysis of peripheral-blood lymphocytes in severe asthma
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کلمات کلیدی
TNFPBMCmRNART-PCRFEV1ICSRMAcRNAcDNA - cDNAComplementary DNA - DNA تکمیلیmessenger RNA - RNA messengercomplementary RNA - RNA مکملEDTA - اتیلن دی آمین تترا استیک اسید ethylenediaminetetraacetate - اتیلنیدامین تتراسیتinterleukin - اینترلوکینtransforming growth factor - تبدیل فاکتور رشدPeripheral-blood mononuclear cell - سلول تک هسته ای محیطی-خونtumor necrosis factor - فاکتور نکروز تومورreal-time polymerase chain reaction - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیInhaled corticosteroid - کورتیکواستروئیدهای استنشاقی
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
پزشکی و دندانپزشکی (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Oligonucleotide-microarray analysis of peripheral-blood lymphocytes in severe asthma Oligonucleotide-microarray analysis of peripheral-blood lymphocytes in severe asthma](/preview/png/9296487.png)
چکیده انگلیسی
CD4+ lymphocytes play a key role in asthma pathogenesis, but much remains unknown about the genetic mechanisms that affect disease severity. In this study we sought to investigate global patterns of gene expression in CD4+ lymphocytes isolated from subjects with severe asthma through the use of microarray technology. CD4+ lymphocytes were separated from peripheral blood, total RNA was purified, and biotinylated complementary RNA was prepared and hybridized to Affymetrix HU133 chips (Affymetrix, Santa Clara, Calif). Using the robust multi-chip average procedure, we compared the messenger RNA expression profiles of more than 33,000 genes of CD4+ lymphocytes in subjects with severe (n = 5) and mild (n = 5) asthma. Forty genes had 2-fold mean expression differences or greater. Thirty-seven genes were up-regulated, including transforming growth factor-β and those involved in T-cell activation, proliferation, and cytoskeletal changes. Three genes were down-regulated, including the T-cell-receptor delta locus. This study demonstrates a method by which CD4+ lymphocytes can be extracted from blood for the purpose of microarray analysis. Furthermore, we show that T-lymphocytes from the peripheral blood of subjects with severe and mild asthma differ in their gene-expression profiles, supporting the view that asthma is a systemic disease. These differentially expressed genes identify potential molecular targets for preventive and therapeutic options for severe asthma.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Laboratory and Clinical Medicine - Volume 145, Issue 5, May 2005, Pages 263-274
Journal: Journal of Laboratory and Clinical Medicine - Volume 145, Issue 5, May 2005, Pages 263-274
نویسندگان
Nadia N. Hansel, Sara C. Hilmer, Steve N. Georas, Leslie M. Cope, Jia Guo, Rafael A. Irizarry, Gregory B. Diette,