کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9353852 | 1604643 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Chemokine IL-8 induction by particulate wear debris in osteoblasts is mediated by NF-κB
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کلمات کلیدی
MCP-1TGF-βc-Jun-NH2-terminal kinaseNF-κBERKIL-1JnkTNFαMAPK - MAPKOsteoblast - استئوبلاست interleukin 1 - اینترلوکین 1transforming growth factor-β - تبدیل فاکتور رشد βtumor necrosis factor-α - تومور نکروز عامل αTitanium - تیتانیومSignaling mechanism - مکانیزم سیگنالینگmonocyte chemoattractant protein-1 - پروتئین شیمیایی monocyte chemoattractant-1mitogen-activated protein kinase - پروتئین کیناز فعال با mitogenextracellular signal-regulated kinase - کیناز تنظیم شده سیگنال خارج سلولی
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
ارتوپدی، پزشکی ورزشی و توانبخشی
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چکیده انگلیسی
Chemokines, or chemotactic cytokines, are major regulators of the inflammatory response and have been identified as pathogenic factors in the periprosthetic soft tissue. Particulate wear debris induced NF-κB activation, the major transcriptional regulator of IL-8 and MCP-1 pro-inflammatory genes and, indeed, both IL-8 and MCP-1 chemokine gene expressions were upregulated in titanium particulate-stimulated human osteoblasts. Here, we demonstrate that phagocytosed particles activate the IL-8 gene promoter via a NF-κB-mediated mechanism. Transfection of a dominant negative mutant IκBα protein that cannot be serine phosphorylated led to suppression of IL-8 promoter activity. The p65/RelA NF-κB subunit activity was affected in both a time- and titanium particle concentration-dependent fashion. Titanium particles led to increased ERK, JNK, and p38 activation in MG-63 osteoblast cells, and IL-8 protein release was suppressed by specific inhibitors of the ERK and p38 MAPK pathways. Together, our results suggest that wear debris particles induce chemokine expression in osteoblasts via NF-κB-mediated transcriptional activation, which is controlled by the MAPK signal transduction pathway.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Orthopaedic Research - Volume 23, Issue 6, November 2005, Pages 1249-1257
Journal: Journal of Orthopaedic Research - Volume 23, Issue 6, November 2005, Pages 1249-1257
نویسندگان
Elizabeth A. Fritz, Joshua J. Jacobs, Tibor T. Glant, Kenneth A. Roebuck,