کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9410674 | 1613319 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Candesartan prevents angiotensin II-induced facilitation of hypoxic neuronal damage through PKCδ inhibition
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کلمات کلیدی
ACEDMEMANGIIPKCδPC12Dulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoAngiotensin-converting enzyme - آنژیوتانسین تبدیل آنزیمAngiotensin II - آنژیوتانسین دوDisorders of the nervous system - اختلالات سیستم عصبیIschemia - ایسکمیDNA fragmentation - تقسیم DNATUNEL - تونلPC12 cell - سلول PC12Rat pheochromocytoma cells - سلولهای فئوکروموسیتوما موش صحراییHypoxia - هیپوکسیprotein kinase Cδ - پروتئین کیناز CδAT1 receptor - گیرنده AT1
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب سلولی و مولکولی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
To investigate the role of protein kinase Cδ (PKCδ) in angiotensin II-induced facilitation mechanisms of hypoxic neuronal damage and whether candesartan, an AT1 receptor antagonist, can suppress these mechanisms, we performed in vitro experiments which were free from vascular components using PC12 cells under hypoxic (12 h)/reoxygenation (0-48 h) conditions. Angiotensin II apparently increased the basal expression level of PKCδ phosphorylated at Ser643 before hypoxia, promoted the cleavage of PKCδ to its catalytic fragment, and fostered the progression of DNA fragmentation after hypoxia. Candesartan inhibited both phosphorylation and cleavage of PKCδ and suppressed the angiotensin II-induced facilitation of DNA fragmentation under hypoxic/reoxygenation conditions. However, PD123319, an AT2 receptor antagonist, influenced neither PKCδ nor the angiotensin II-induced facilitation of DNA fragmentation. Furthermore, in PC12 cells expressing the ATP-binding mutant of PKCδ (PKCδK376R) acting as a dominant-negative protein, both phosphorylation and cleavage of PKCδ were attenuated and DNA fragmentation was markedly suppressed regardless of the presence of angiotensin II. These findings suggest that angiotensin II-induced facilitation of DNA fragmentation under hypoxic conditions is mediated by PKCδ, and the mechanisms can be suppressed by the candesartan mediated blockade of the AT1 receptor.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Brain Research - Volume 135, Issues 1â2, 27 April 2005, Pages 134-140
Journal: Molecular Brain Research - Volume 135, Issues 1â2, 27 April 2005, Pages 134-140
نویسندگان
Kimiaki Utsugisawa, Yuriko Nagane, Taiju Utsugisawa, Daiji Obara, Yasuo Terayama,