کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9410774 | 1613317 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Alternative splicing and promoter use in the human GABRA2 gene
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کلمات کلیدی
Promoter functionγ-aminobutyric acid - اسید γ-آمینوبوتیریکNeurotransmitters, Modulators, Transporters, and Receptors - انتقال دهنده های عصبی، مدولاتورها، حمل کننده ها و گیرنده هاTranscriptional regulation - تنظیم ترانزیتیAlternative splicing - جابجایی جایگزینneurotransmitter receptor - گیرنده نوروترانسمیترGABAA receptors - گیرنده های GABAA
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب سلولی و مولکولی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Alternative splicing and promoter use in the human GABRA2 gene Alternative splicing and promoter use in the human GABRA2 gene](/preview/png/9410774.png)
چکیده انگلیسی
GABAA receptors mediate the majority of the fast synaptic inhibition in the mammalian brain. They are the targets of several important drugs, including benzodiazepines, which are used as anxiolytics, sedatives, anti-convulsants, and in the treatment of alcohol withdrawal symptoms. Non-coding variations in GABRA2, the gene encoding the α2 subunit, are associated with the risk for alcoholism, suggesting that regulatory differences are important. GABRA2 mRNAs from whole human brain and from three brain regions were examined for evidence of alternative splicing using reverse transcription-PCR and DNA sequencing. A complex pattern of alternative splicing and alternative promoter use of the human GABRA2 mRNA was demonstrated. There are four major isoforms consisting of combinations of two alternative 5Ⲡand 3Ⲡexons, as well as minor isoforms lacking exon 4 or exon 8. The alternative 5Ⲡexons each lie downstream of a functional promoter sequence, as shown by transient transfection assays. The promoter activities of naturally occurring haplotypes differed, indicating genetic differences in gene expression.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Brain Research - Volume 137, Issues 1â2, 13 June 2005, Pages 174-183
Journal: Molecular Brain Research - Volume 137, Issues 1â2, 13 June 2005, Pages 174-183
نویسندگان
Huijun Tian, Hui-Ju Chen, Tiffeny H. Cross, Howard J. Edenberg,