کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9416341 | 1614332 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Neuroprotective effect of N-acetyl cysteine on hypoxia-induced oxidative stress in primary hippocampal culture
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کلمات کلیدی
GPXMMPPBSNACGSHGSSGSDSN-acetyl l-cysteinePrimary hippocampal cultureDCFH-DAO−2 - O-2ROS - ROSHydrogen peroxide - آب اکسیژنهsuperoxide anion - آنیون سوپر اکسیدEDTA - اتیلن دی آمین تترا استیک اسید Ethylene diamine tetra acetic acid - اتیلن دیامین تترا استیک اسیدDNA fragmentation - تقسیم DNAOxidative stress - تنش اکسیداتیوCellular and molecular biology - زیست شناسی سلولی و مولکولیsodium dodecyl sulphate - سدیم دودسیل سولفاتCytotoxicity - سمیت سلولیPhosphate buffered saline - فسفات بافر شورlactate dehydrogenase - لاکتات دهیدروژناز LDH - لاکتات دهیدروژناز به صورت مختصر شده LDH Nitric oxide - نیتریک اکسیدH2O2 - هیدروژن پراکسیدAntioxidant status - وضعیت آنتیاکسیدانیMitochondrial membrane potential - پتانسیل غشای میتوکندریGlutathione - گلوتاتیونoxidised glutathione - گلوتاتیون اکسید شدهReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Hippocampus has received a considerable attention in the recent past due to its role in a number of important functions such as learning and memory. The effect of hypoxia on neuronal cell injury especially on hippocampal cells is not well known. The aim of the present study was to characterize the biochemical changes in primary cultured hippocampal neurons during hypoxic exposure and the protective effect of N-acetyl cysteine on hypoxia-induced cytotoxicity. The hippocampal culture grown in 24-well plates was exposed to hypoxia for 3 h in a dessicator in 95% N2, 5% CO2 atmosphere at 37 °C. Later, the cells were allowed to recover for 1 h under normoxia. It was observed that there is an appreciable increase in cytotoxicity in cells exposed to hypoxia. Further, there was a significant decrease in mitochondrial membrane potential and appreciable increase in reactive oxygen species and single-strand DNA breaks in cells exposed to hypoxia compared to control. There is a significant fall in glutathione peroxidase, glutathione reductase, reduced glutathione levels, and nitric oxide in the cells exposed to hypoxia. Significant elevation in the intracellular calcium level in the cells on exposure to hypoxia was observed. Supplementation with NAC (50 μM) resulted in a significant cytoprotection, fall in ROS generation, and higher antioxidant levels similar to that of control cells. NAC also inhibited DNA strand breaks induced by hypoxia. The study indicates that NAC has significant neuroprotective activity during hypoxia in primary hippocampal culture.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1046, Issues 1â2, 7 June 2005, Pages 97-104
Journal: Brain Research - Volume 1046, Issues 1â2, 7 June 2005, Pages 97-104
نویسندگان
K. Jayalakshmi, M. Sairam, S.B. Singh, S.K. Sharma, G. Ilavazhagan, P.K. Banerjee,