کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9422687 | 1294693 | 2005 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Reanalysis of the protocol for in vitro synchronization of mammalian astrocytic cultures by serum deprivation
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
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چکیده انگلیسی
Serum starvation of astrocytes for a period of time followed by refeeding has been proposed as a method to produce synchronized astrocytes. Here, it is proposed that the method neither synchronizes cells nor satisfies rigorous criteria for cell synchronization. The proposed non-selective, whole-culture synchronization protocol cannot, in theory, synchronize cells. The cells produced by the proposed serum starvation/refeeding protocol do not reflect the properties of any particular cell during the cell cycle. Cells produced by the published protocol will have a wide distribution of cell sizes, and therefore, the cells produced by starvation/refeeding will not model cells of any specific age during the division cycle. Thus, the proposed protocol will not produce a synchronized culture.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research Protocols - Volume 15, Issue 3, September 2005, Pages 115-118
Journal: Brain Research Protocols - Volume 15, Issue 3, September 2005, Pages 115-118
نویسندگان
Stephen Cooper,