کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9425469 | 1295874 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Structural difference between heteromeric somatic and homomeric axonal glycine receptors in the hypothalamo-neurohypophysial system
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کلمات کلیدی
SDSethylene glycol-bis-(2-aminoethylether)-N,N,N′,N′-tetraacetic acidHNSNeurohypophysisGlyRRT-PCREGTAPBSEDTA - اتیلن دی آمین تترا استیک اسید ethylenediamine-tetraacetic acid - اتیلندیامین-تتراستیک اسیدoxytocin - اکسیتوسینTaurine - تائورینsodium dodecyl sulfate - سدیم دودسیل سولفاتNeuroendocrine cells - سلولهای نوروآندوکرینhypothalamo-neurohypophysial system - سیستم هیپوتالامو- نوروپاتی فیزیکیSON - فرزند پسرPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریReceptor targeting - هدف گیرندهSupraoptic nucleus - هسته SupraopticHypothalamus - هیپوتالاموسvasopressin - وازوپرسینreverse transcription–polymerase chain reaction - واکنش زنجیره ای رونویسی-پلیمراز معکوسPresynaptic receptors - گیرنده های پیشینپتیکglycine receptor - گیرنده گلیسین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Structural difference between heteromeric somatic and homomeric axonal glycine receptors in the hypothalamo-neurohypophysial system Structural difference between heteromeric somatic and homomeric axonal glycine receptors in the hypothalamo-neurohypophysial system](/preview/png/9425469.png)
چکیده انگلیسی
Glycine receptors are ionotropic receptors formed by either the homomeric assembly of ligand-binding α subunits or the heteromeric combination of an α subunit and the auxiliary β subunit. Glycine receptors in the brain are found at either pre- or post-synaptic sites. Rat supraoptic nucleus neurons express glycine receptors on the membrane of both their soma and dendrites within the supraoptic nucleus, and their axon terminals in the neurohypophysis. Taking advantage of the well-separated cellular compartments of this system, we correlated the structural properties of the receptors to their subcellular localization. Immunohistochemical study using the generic mAb4a antibody revealed that somatodendritic receptors were clustered, whereas axonal glycine receptors showed a more diffuse distribution. This was paralleled by the presence of clusters of the glycine receptor aggregating protein gephyrin in the supraoptic nucleus and its complete absence in the neurohypophysis. Moreover, another antibody recognizing the α1/α2 subunits similarly labeled the axonal glycine receptors, but did not recognize the somatodendritic receptor clusters of supraoptic nucleus neurons, indicative of structural differences between somatic and axonal glycine receptors. Furthermore, the subunits composing the somatic and axonal receptors have different molecular weight. Functional study further differentiated the two types of glycine receptors on the basis of their sensitivity to picrotoxin, identifying somatic receptors as α/β heteromers, and axonal receptors as α homomers. These results indicate that targeting of glycine receptors to axonal or somatodendritic compartment is directly related to their subunit composition, and set the hypothalamo-neurohypophysial system as an excellent model to study the mechanisms of targeting of proteins to various neuronal cellular compartments.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 135, Issue 2, 2005, Pages 475-483
Journal: Neuroscience - Volume 135, Issue 2, 2005, Pages 475-483
نویسندگان
C. Deleuze, M. Runquist, H. Orcel, A. Rabié, G. Dayanithi, G. Alonso, N. Hussy,