کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9425532 | 1295878 | 2005 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression of estrogen receptor alpha exon-deleted mRNA variants in the human and non-human primate frontal cortex
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کلمات کلیدی
ERαIsoform - ایسوفورGene expression - بیان ژنReverse transcriptase - ترانس کریپتاز معکوس یا وارونویسbase pair - جفت پایهPMI - شرکتهای کوچک و متوسطPostmortem interval - فاصله زمانی PostmortemBrain - مغزNational Institute of Mental Health - موسسه ملی سلامت روانMonkey - میمونNIMH - نیمهHormone - هورمونpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازSplicing - چسباندنEstrogen receptor alpha - گیرنده استروژن آلفا
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Although estrogen receptor alpha (ERα) mRNA has been detected in the primate frontal cortex, the types of ERα transcripts expressed, including exon-deleted variants (Î), have not been determined in the monkey or human frontal cortex. Because the types of ERα mRNA expressed in brain could define neuronal responses to estrogens, we examined the transcript pool of ERα mRNAs expressed in normal adult and developing human and macaque frontal cortex. We reverse transcribed total RNA from the postmortem frontal cortex of 29 normal adult humans, 12 rhesus macaques, and 19 people ranging from infants to adults and employed two rounds of nested polymerase chain reaction (PCR) to generate ERα products spanning the coding domain. In a third nested PCR, we used primers specific for novel sequences of exon-exon junctions created when whole exons are missing. By sequencing PCR products, we detected 60 instances of 12 distinct ÎERα mRNAs in adult humans and 94 instances of 13 distinct ÎERα mRNAs in monkeys in differing patterns from one individual to another. In adult humans, 83% of individuals expressed at least 1 ÎERα mRNA variant, and 100% of the monkeys expressed at least 1 ÎERα mRNA variant. The single Î2, Î5, and Î7 variants were frequently expressed in both human and monkey frontal cortex, Î3 variants were rare in both species, and Î6 variants were more frequently expressed in monkeys. In both species, we detected double, triple and quadruple Îs, but these were less common than single Îs. The pattern of human variant expression did not appear to change dramatically as a function of age. These findings imply the potential to produce different ERα proteins in frontal cortex, possibly with altered structure and function which may have physiological relevance for gene transcription by virtue of altered functional interactions with each other, other steroid hormone receptors, and genomic DNA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 134, Issue 1, 2005, Pages 81-95
Journal: Neuroscience - Volume 134, Issue 1, 2005, Pages 81-95
نویسندگان
W.R. Perlman, M. Matsumoto, S. Beltaifa, T.M. Hyde, R.C. Saunders, M.J. Webster, D.R. Rubinow, J.E. Kleinman, C.S. Weickert,